Key features and details
- Rabbit polyclonal to Tankyrase
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Tankyrase antibody
See all Tankyrase primary antibodies
DescriptionRabbit polyclonal to Tankyrase
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
- 721_B cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 2% Sucrose
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab86279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 142 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
FunctionMay regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles. Has PARP activity and can modify TERF1, and thereby contribute to the regulation of telomere length.
Tissue specificityUbiquitous; highest levels in testis.
Sequence similaritiesContains 15 ANK repeats.
Contains 1 PARP catalytic domain.
Contains 1 SAM (sterile alpha motif) domain.
modificationsPhosphorylated on serine residues by MAPK kinases upon insulin stimulation.
Cellular localizationCytoplasm. Golgi apparatus membrane. Chromosome > centromere. Nucleus > nuclear pore complex. Chromosome > telomere. Associated with the Golgi and with juxtanuclear SLC2A4/GLUT4-vesicles. A minor proportion is also found at nuclear pore complexes and around the pericentriolar matrix of mitotic centromeres. During interphase, a small fraction of TNKS is found in the nucleus, associated with TERF1.
- Information by UniProt
- ADP-ribosyltransferase diphtheria toxin-like 5 antibody
- OTTHUMP00000115550 antibody
- OTTHUMP00000224675 antibody
Anti-Tankyrase antibody (ab86279) at 1 µg/ml (5% skim milk/ PBS buffer) + 721_B cell lysate at 10 µg with gel concentration: 6-18%
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 142 kDa
Observed band size: 142 kDa
Additional bands at: <40 kDa. We are unsure as to the identity of these extra bands.
ab86279 has been referenced in 2 publications.
- Gu M et al. RhoA phosphorylation mediated by Rho/RhoA-associated kinase pathway improves the anti-freezing potentiality of murine hatched and diapaused blastocysts. Sci Rep 7:6705 (2017). PubMed: 28751650
- DaRosa PA et al. Allosteric activation of the RNF146 ubiquitin ligase by a poly(ADP-ribosyl)ation signal. Nature 517:223-6 (2015). PubMed: 25327252