• Product name

  • Description

    Rabbit polyclonal to Tankyrase
  • Host species

  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within the internal amino acids 1080-1129 (


    ) of Human Tankyrase (NP_003738).

  • Positive control

    • 721_B cell lysate


  • Form

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 2% Sucrose
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab86279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 142 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • Function

    May regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles. Has PARP activity and can modify TERF1, and thereby contribute to the regulation of telomere length.
  • Tissue specificity

    Ubiquitous; highest levels in testis.
  • Sequence similarities

    Contains 15 ANK repeats.
    Contains 1 PARP catalytic domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Post-translational

    Phosphorylated on serine residues by MAPK kinases upon insulin stimulation.
    ADP-ribosylated (-auto).
  • Cellular localization

    Cytoplasm. Golgi apparatus membrane. Chromosome > centromere. Nucleus > nuclear pore complex. Chromosome > telomere. Associated with the Golgi and with juxtanuclear SLC2A4/GLUT4-vesicles. A minor proportion is also found at nuclear pore complexes and around the pericentriolar matrix of mitotic centromeres. During interphase, a small fraction of TNKS is found in the nucleus, associated with TERF1.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADP-ribosyltransferase diphtheria toxin-like 5 antibody
    • OTTHUMP00000115550 antibody
    • OTTHUMP00000224675 antibody
    • PARP 5a antibody
    • PARP5A antibody
    • PARPL antibody
    • pART5 antibody
    • Poly [ADP-ribose] polymerase 5A antibody
    • TANK 1 antibody
    • TANK1 antibody
    • Tankyrase 1 antibody
    • Tankyrase I antibody
    • Tankyrase TRF1 interacting ankyrin related ADP ribose polymerase antibody
    • Tankyrase-1 antibody
    • Tankyrase1 antibody
    • TankyraseI antibody
    • TIN 1 antibody
    • TIN1 antibody
    • TINF 1 antibody
    • TINF1 antibody
    • TNKS 1 antibody
    • TNKS antibody
    • TNKS-1 antibody
    • TNKS1 antibody
    • TNKS1_HUMAN antibody
    • TRF1 interacting ankyrin related ADP ribose polymerase antibody
    • TRF1-interacting ankyrin-related ADP-ribose polymerase antibody
    see all


  • Anti-Tankyrase antibody (ab86279) at 1 µg/ml (5% skim milk/ PBS buffer) + 721_B cell lysate at 10 µg with gel concentration: 6-18%

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 142 kDa
    Observed band size: 142 kDa
    Additional bands at: <40 kDa. We are unsure as to the identity of these extra bands.


This product has been referenced in:

  • Gu M  et al. RhoA phosphorylation mediated by Rho/RhoA-associated kinase pathway improves the anti-freezing potentiality of murine hatched and diapaused blastocysts. Sci Rep 7:6705 (2017). Read more (PubMed: 28751650) »
  • DaRosa PA  et al. Allosteric activation of the RNF146 ubiquitin ligase by a poly(ADP-ribosyl)ation signal. Nature 517:223-6 (2015). Read more (PubMed: 25327252) »
See all 2 Publications for this product

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