Overview

  • Product name

    Anti-Tankyrase antibody - C-terminal
    See all Tankyrase primary antibodies
  • Description

    Rabbit polyclonal to Tankyrase - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Tankyrase (C terminal). The exact sequence is proprietary.
    Database link: O95271

  • Positive control

    • WB: MCF7 cells. IHC-P: SCM1 xenograft tissue. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 142 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    May regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles. Has PARP activity and can modify TERF1, and thereby contribute to the regulation of telomere length.
  • Tissue specificity

    Ubiquitous; highest levels in testis.
  • Sequence similarities

    Contains 15 ANK repeats.
    Contains 1 PARP catalytic domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Post-translational
    modifications

    Phosphorylated on serine residues by MAPK kinases upon insulin stimulation.
    ADP-ribosylated (-auto).
  • Cellular localization

    Cytoplasm. Golgi apparatus membrane. Chromosome > centromere. Nucleus > nuclear pore complex. Chromosome > telomere. Associated with the Golgi and with juxtanuclear SLC2A4/GLUT4-vesicles. A minor proportion is also found at nuclear pore complexes and around the pericentriolar matrix of mitotic centromeres. During interphase, a small fraction of TNKS is found in the nucleus, associated with TERF1.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADP-ribosyltransferase diphtheria toxin-like 5 antibody
    • OTTHUMP00000115550 antibody
    • OTTHUMP00000224675 antibody
    • PARP 5a antibody
    • PARP5A antibody
    • PARPL antibody
    • pART5 antibody
    • Poly [ADP-ribose] polymerase 5A antibody
    • TANK 1 antibody
    • TANK1 antibody
    • Tankyrase 1 antibody
    • Tankyrase I antibody
    • Tankyrase TRF1 interacting ankyrin related ADP ribose polymerase antibody
    • Tankyrase-1 antibody
    • Tankyrase1 antibody
    • TankyraseI antibody
    • TIN 1 antibody
    • TIN1 antibody
    • TINF 1 antibody
    • TINF1 antibody
    • TNKS 1 antibody
    • TNKS antibody
    • TNKS-1 antibody
    • TNKS1 antibody
    • TNKS1_HUMAN antibody
    • TRF1 interacting ankyrin related ADP ribose polymerase antibody
    • TRF1-interacting ankyrin-related ADP-ribose polymerase antibody
    see all

Images

  • Anti-Tankyrase antibody - C-terminal (ab227471) at 1/500 dilution + MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 142 kDa



    5% SDS-PAGE

  • Paraffin-embedded SCM1 xenograft tissue stained for Tankyrase with ab227471 at 1/100 dilution in immunohistochemical analysis.

  • Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Tankyrase (green) using ab227471 at 1/500 dilution in ICC/IF.

    Alpha-tubulin filaments were labeled with an anti-Alpha-Tubulin antibody at 1/200 dilution (red).

References

This product has been referenced in:

  • Yang HY  et al. Tankyrase Promotes Aerobic Glycolysis and Proliferation of Ovarian Cancer through Activation of Wnt/ß-Catenin Signaling. Biomed Res Int 2019:2686340 (2019). Read more (PubMed: 30915350) »
See 1 Publication for this product

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