Key features and details
- Rabbit polyclonal to Tankyrase - N-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Tankyrase antibody - N-terminal
See all Tankyrase primary antibodies
DescriptionRabbit polyclonal to Tankyrase - N-terminal
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Rhesus monkey
Recombinant fragment within Human Tankyrase (N terminal). The exact sequence is proprietary.
Database link: O95271
- ICC/IF: MCF7 cells. IHC-P: Human hepatoma tissue. WB: U-87 MG whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 142 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionMay regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles. Has PARP activity and can modify TERF1, and thereby contribute to the regulation of telomere length.
Tissue specificityUbiquitous; highest levels in testis.
Sequence similaritiesContains 15 ANK repeats.
Contains 1 PARP catalytic domain.
Contains 1 SAM (sterile alpha motif) domain.
modificationsPhosphorylated on serine residues by MAPK kinases upon insulin stimulation.
Cellular localizationCytoplasm. Golgi apparatus membrane. Chromosome > centromere. Nucleus > nuclear pore complex. Chromosome > telomere. Associated with the Golgi and with juxtanuclear SLC2A4/GLUT4-vesicles. A minor proportion is also found at nuclear pore complexes and around the pericentriolar matrix of mitotic centromeres. During interphase, a small fraction of TNKS is found in the nucleus, associated with TERF1.
- Information by UniProt
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Anti-Tankyrase antibody - N-terminal (ab227469) at 1/1000 dilution + U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg
Predicted band size: 142 kDa
5% SDS-PAGE gel.
Paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for Tankyrase (green) using ab227469 at 1/200 dilution in ICC/IF.
Counterstain: Hoechst 33342 (blue).
Paraffin-embedded human hepatoma tissue stained for Tankyrase using ab227469 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227469 has not yet been referenced specifically in any publications.