Anti-Tankyrase binding protein 1 antibody - N-terminal (ab155498)
Key features and details
- Rabbit polyclonal to Tankyrase binding protein 1 - N-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Tankyrase binding protein 1 antibody - N-terminal
See all Tankyrase binding protein 1 primary antibodies -
Description
Rabbit polyclonal to Tankyrase binding protein 1 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to a region within N terminal amino acids 1-44 of Human Tankyrase binding protein 1 (Uniprot ID: Q9C0C2).
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Positive control
- A431, H1299 whole cell lysate; A431 cells; Human brain cortex tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155498 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 182 kDa.
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IHC-P |
1/100 - 1/1000. Suggested antigen retrieval
using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)].
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 182 kDa. |
IHC-P
1/100 - 1/1000. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. |
ICC/IF
1/100 - 1/1000. |
Target
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Tissue specificity
Detected in testis, ovary, lung, skeletal muscle, heart, prostate and pancreas, and at very low levels in brain and peripheral blood leukocytes. -
Post-translational
modificationsADP-ribosylated by TNKS1 (in vitro).
Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Cytoplasm > cytoskeleton. Chromosome. Colocalizes with chromosomes during mitosis, and in the cytoplasm with cortical actin. - Information by UniProt
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Database links
- Entrez Gene: 85456 Human
- Omim: 607104 Human
- SwissProt: Q9C0C2 Human
- Unigene: 530730 Human
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Alternative names
- 182 kDa tankyrase 1 binding protein antibody
- 182 kDa tankyrase-1-binding protein antibody
- FLJ45975 antibody
see all
Images
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All lanes : Anti-Tankyrase binding protein 1 antibody - N-terminal (ab155498) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 182 kDa
5% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human brain cortex tissue labeling Tankyrase binding protein 1 with ab155498 at 1/100 dilution.
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Immunofluorescent analysis of methanol-fixed A431 cells labeling Tankyrase binding protein 1 with ab155498 at 1/200 dilution. Lower panel co-stained with Hoescht 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab155498 has been referenced in 1 publication.
- Cao Y et al. Elevated TAB182 enhances the radioresistance of esophageal squamous cell carcinoma through G2-M checkpoint modulation. Cancer Med 10:3101-3112 (2021). PubMed: 33787085