Anti-TAOK2 antibody (ab155595)
Key features and details
- Rabbit polyclonal to TAOK2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TAOK2 antibody
See all TAOK2 primary antibodies -
Description
Rabbit polyclonal to TAOK2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human TAOK2 aa 69-350 (N terminal).
Database link: Q9UL54 -
Positive control
- Mouse brain whole cell lysate; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155595 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 138 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 138 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Isoform 1, but not isoform 2, plays a role in apoptotic morphological changes, including cell contraction, membrane blebbing and apoptotic bodies formation. This function, which requires the activation of MAPK8/JNK and nuclear localization of C-terminally truncated isoform 1, may be linked to the mitochondrial CASP9-associated death pathway. Isoform 1, but not isoform 2, activates the JNK MAP kinase pathway through the specific activation of the upstream MKK3 and MKK6 kinases. Isoform 1 binds to microtubules and affects their organization and stability independently of its kinase activity. Prevents MAP3K7-mediated activation of IKKA, and thus NF-kappa-B activation, but not that of JNK. Phosphorylates itself, MBP, activated MAPK8 and tubulins. May play a role in the osmotic stress-MAPK8 pathway. Isoform 2, but not isoform 1, is required for PCDH8 endocytosis. Following homophilic interactions between PCDH8 extracellular domains, isoform 2 phosphorylates and activates MAPK14/p38 MAPK which in turn phosphorylates isoform 2. This process leads to PCDH8 endocytosis and CDH2 cointernalization (By similarity). Both isoforms are involved in MAPK14 phosphorylation. -
Tissue specificity
Ubiquitously expressed, with a higher level of expression in testis and brain. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsIsoforms 1 and 2 are autophosphorylated.
C-terminal cleavage of isoform 1 and subsequent nuclear localization requires CASP9 activity.
isoform 2 is phosphorylated at 'Ser-1031' by MAPK14. This phosphorylation is required PCDH8 for endocytosis. -
Cellular localization
Cytoplasmic vesicle membrane. Cytoplasm > cytoskeleton. Nucleus. Catalytically active full-length phosphorylated isoform 1 localizes to microtubules in the cytoplasm predominantly on microtubule cables positioned around the nucleus. A C-terminally truncated form of isoform 1 is present in the nucleus; isoform 2 and kinase-defective, as well as full-length isoform 1 are excluded from the nucleus and Cell projection > dendrite. In dendrites, colocalizes with PCDH8. - Information by UniProt
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Database links
- Entrez Gene: 9344 Human
- Entrez Gene: 381921 Mouse
- Omim: 613199 Human
- SwissProt: Q9UL54 Human
- SwissProt: Q6ZQ29 Mouse
- Unigene: 291623 Human
- Unigene: 259634 Mouse
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Alternative names
- 1110033K02Rik antibody
- B230344N16 antibody
- hKFC C antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab155595 has not yet been referenced specifically in any publications.