Key features and details
- Rabbit polyclonal to TAOK2
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-TAOK2 antibody
See all TAOK2 primary antibodies
DescriptionRabbit polyclonal to TAOK2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Cow, Human, Xenopus laevis, Xenopus tropicalis
Recombinant fragment, corresponding to a region within N terminal amino acids 3-285 of Human TAO kinase 2 (Uniprot ID Q9UL54).
- Mouse brain whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab155603 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 138 kDa.|
FunctionIsoform 1, but not isoform 2, plays a role in apoptotic morphological changes, including cell contraction, membrane blebbing and apoptotic bodies formation. This function, which requires the activation of MAPK8/JNK and nuclear localization of C-terminally truncated isoform 1, may be linked to the mitochondrial CASP9-associated death pathway. Isoform 1, but not isoform 2, activates the JNK MAP kinase pathway through the specific activation of the upstream MKK3 and MKK6 kinases. Isoform 1 binds to microtubules and affects their organization and stability independently of its kinase activity. Prevents MAP3K7-mediated activation of IKKA, and thus NF-kappa-B activation, but not that of JNK. Phosphorylates itself, MBP, activated MAPK8 and tubulins. May play a role in the osmotic stress-MAPK8 pathway. Isoform 2, but not isoform 1, is required for PCDH8 endocytosis. Following homophilic interactions between PCDH8 extracellular domains, isoform 2 phosphorylates and activates MAPK14/p38 MAPK which in turn phosphorylates isoform 2. This process leads to PCDH8 endocytosis and CDH2 cointernalization (By similarity). Both isoforms are involved in MAPK14 phosphorylation.
Tissue specificityUbiquitously expressed, with a higher level of expression in testis and brain.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain.
modificationsIsoforms 1 and 2 are autophosphorylated.
C-terminal cleavage of isoform 1 and subsequent nuclear localization requires CASP9 activity.
isoform 2 is phosphorylated at 'Ser-1031' by MAPK14. This phosphorylation is required PCDH8 for endocytosis.
Cellular localizationCytoplasmic vesicle membrane. Cytoplasm > cytoskeleton. Nucleus. Catalytically active full-length phosphorylated isoform 1 localizes to microtubules in the cytoplasm predominantly on microtubule cables positioned around the nucleus. A C-terminally truncated form of isoform 1 is present in the nucleus; isoform 2 and kinase-defective, as well as full-length isoform 1 are excluded from the nucleus and Cell projection > dendrite. In dendrites, colocalizes with PCDH8.
- Information by UniProt
- 1110033K02Rik antibody
- B230344N16 antibody
- hKFC C antibody
ab155603 has not yet been referenced specifically in any publications.