Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4883] to TAOK3 (phospho S177) + TAOK2 (phospho S181) + TAOK1 (phospho S181)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Product nameAnti-TAOK3 (phospho S177) + TAOK2 (phospho S181) + TAOK1 (phospho S181) antibody [EPR4883]
DescriptionRabbit monoclonal [EPR4883] to TAOK3 (phospho S177) + TAOK2 (phospho S181) + TAOK1 (phospho S181)
Specificityab124841 recognizes Human TAOK1 when phosphorylated at Serine 181, TAOK2 when phosphorylated at Serine 181 and TAOK3 when phosphorylated at Serine 177.
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human TAOK1. The exact sequence is proprietary. Synthetic peptide corresponding to residues surrounding Serine 181 of Human TAO kinase 2 (Q9UL54) or Serine 181 of TAO kinase 1 (Q7L7X3) or Serine 177 of TAO kinase 3 (Q9H2K8).
- 293T cell lysates and Human colonic adenocarcinoma tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124841 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 138 kDa.|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended.
Cellular localizationTAOK3: Cytoplasm. Cell membrane. Also localized to the peripheral cell membrane. TAOK2: Cytoplasmic vesicle membrane. Cytoplasm > cytoskeleton. Nucleus. Catalytically active full-length phosphorylated isoform 1 localizes to microtubules in the cytoplasm predominantly on microtubule cables positioned around the nucleus. A C-terminally truncated form of isoform 1 is present in the nucleus; isoform 2 and kinase-defective, as well as full-length isoform 1 are excluded from the nucleus and Cell projection > dendrite. In dendrites, colocalizes with PCDH8. TAOK1: Cytoplasm.
All lanes : Anti-TAOK3 (phospho S177) + TAOK2 (phospho S181) + TAOK1 (phospho S181) antibody [EPR4883] (ab124841) at 1/1000 dilution
Lane 1 : Untreated 293T cell lysates
Lane 2 : Lambda Phosphatase treated 293T cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 138 kDa
ab124841, at 1/250 to 1/500, staining TAOK3 (phospho S177) + TAOK2 (phospho S181) + TAOK1 (phospho S181) in Human colonic adenocarcinoma by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab124841 has been referenced in 1 publication.
- Hwang IY et al. Gai Signaling Promotes Marginal Zone B Cell Development by Enabling Transitional B Cell ADAM10 Expression. Front Immunol 9:687 (2018). PubMed: 29696016