Anti-TAP tag antibody [22F12-1E3] - C-terminal (ab185976)

Overview

  • Product name

    Anti-TAP tag antibody [22F12-1E3] - C-terminal
  • Description

    Mouse monoclonal [22F12-1E3] to TAP tag - C-terminal
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Synthetic peptide corresponding to TAP tag (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). KLH conjugated peptide representing the C-terminus of the TAP construct after TEV cleavage (Tobacco Etch Virus)

  • Positive control

    • TAP-tag containing control protein.
  • General notes

    TAP adds about 20 kDa to the size of the protein.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 30% Glycerol, 0.1% BSA, 69% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    22F12-1E3
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab185976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000.

Target

  • Relevance

    Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tandem affinity purification (TAP) is an affinity purification method for isolation of TAP-tagged proteins together with associated proteins. The protocol involves the fusion of the "TAP tag" (typically a calmodulin binding peptide (CBP), a tobacco etch virus protease (TEV protease) cleavage site and Protein A) to the protein of interest. The TAP technique is useful in analyzing in vivo interactions.

Images

  • All lanes : Anti-TAP tag antibody [22F12-1E3] - C-terminal (ab185976) at 1/1000 dilution

    Lane 1 : TAP-tag containing control protein at 20 µl
    Lane 2 : TAP-tag containing control protein at 10 µl
    Lane 3 : TAP-tag containing control protein at 5 µl

    Secondary
    All lanes : goat anti-mouse IgG-HRP at 1/15000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

References

ab185976 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

The antibody ab185976 will detect intact TAP, and will probably also detect the product of TEV protease cleavage of the TAP tag too, though there is no data demonstrating that. The samples for the western blot data on our datasheet contain intact, non-cleaved TAP tag.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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