Key features and details
- Mouse monoclonal [22F12-1E3] to TAP tag - C-terminal
- Suitable for: WB
- Isotype: IgG1
Product nameAnti-TAP tag antibody [22F12-1E3] - C-terminal
DescriptionMouse monoclonal [22F12-1E3] to TAP tag - C-terminal
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Other species
Synthetic peptide corresponding to TAP tag (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). KLH conjugated peptide representing the C-terminus of the TAP construct after TEV cleavage (Tobacco Etch Virus)
- TAP-tag containing control protein.
TAP adds about 20 kDa to the size of the protein.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 0.1% BSA, 69% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab185976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000.|
RelevanceEpitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tandem affinity purification (TAP) is an affinity purification method for isolation of TAP-tagged proteins together with associated proteins. The protocol involves the fusion of the "TAP tag" (typically a calmodulin binding peptide (CBP), a tobacco etch virus protease (TEV protease) cleavage site and Protein A) to the protein of interest. The TAP technique is useful in analyzing in vivo interactions.
All lanes : Anti-TAP tag antibody [22F12-1E3] - C-terminal (ab185976) at 1/1000 dilution
Lane 1 : TAP-tag containing control protein at 20 µl
Lane 2 : TAP-tag containing control protein at 10 µl
Lane 3 : TAP-tag containing control protein at 5 µl
All lanes : goat anti-mouse IgG-HRP at 1/15000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
ab185976 has not yet been referenced specifically in any publications.