Anti-TAP1 antibody [53H8] (ab10356)
Key features and details
- Mouse monoclonal [53H8] to TAP1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-TAP1 antibody [53H8] -
Description
Mouse monoclonal [53H8] to TAP1 -
Host species
Mouse -
Tested Applications & Species
Application Species WB Human -
Immunogen
Unfortunately, this information is considered to be commercially sensitive
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Protein A purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
53H8 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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WB |
Human
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Application | Abreviews | Notes |
---|---|---|
WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 81 kDa (predicted molecular weight: 87 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 81 kDa (predicted molecular weight: 87 kDa). |
Target
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Function
Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Expression of TAP1 is down-regulated by human Epstein-Barr virus vIL-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules. -
Involvement in disease
Defects in TAP1 are a cause of bare lymphocyte syndrome type 1 (BLS1) [MIM:604571]; also called HLA class I deficiency. BLS1 is a class I antigen deficiency that is not accompanied by particular pathologic manifestations during the first years of life. Systemic infections have not been described. Chronic bacterial infections, often beginning in the first decade of life, are restricted to the respiratory tract. -
Sequence similarities
Belongs to the ABC transporter superfamily. ABCB family. MHC peptide exporter (TC 3.A.1.209) subfamily.
Contains 1 ABC transmembrane type-1 domain.
Contains 1 ABC transporter domain. -
Domain
The peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2. -
Cellular localization
Endoplasmic reticulum membrane. The transmembrane segments seem to form a pore in the membrane. - Information by UniProt
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Database links
- Entrez Gene: 6890 Human
- Omim: 170260 Human
- SwissProt: Q03518 Human
- Unigene: 352018 Human
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Alternative names
- ABC 17 antibody
- ABC transporter MHC 1 antibody
- ABC17 antibody
see all
Images
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All lanes : Anti-TAP1 antibody [53H8] (ab10356) at 5 µg/ml
Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 87 kDa
Observed band size: 81 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa (possible non-specific binding)
References (1)
ab10356 has been referenced in 1 publication.
- Mo MS et al. Dopaminergic neurons show increased low-molecular-mass protein 7 activity induced by 6-hydroxydopamine in vitro and in vivo. Transl Neurodegener 7:19 (2018). PubMed: 30128145