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    tap1-antibody-53h8-ab10356.pdf

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Signal Transduction Adapters Transmembrane
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Anti-TAP1 antibody [53H8] (ab10356)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (1)

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Western blot - Anti-TAP1 antibody [53H8] (ab10356)

    Key features and details

    • Mouse monoclonal [53H8] to TAP1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG1

    You may also be interested in

    Protein
    Product image
    Recombinant Human TAP1 protein (ab159637)
    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)

    View more associated products

    Overview

    • Product name

      Anti-TAP1 antibody [53H8]
    • Description

      Mouse monoclonal [53H8] to TAP1
    • Host species

      Mouse
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Unfortunately, this information is considered to be commercially sensitive

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.1% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Purification notes

      Protein A purified from tissue culture supernatant.
    • Clonality

      Monoclonal
    • Clone number

      53H8
    • Myeloma

      Sp2/0
    • Isotype

      IgG1
    • Research areas

      • Signal Transduction
      • Adapters
      • Transmembrane

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant Human TAP1 protein (ab159637)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab10356 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB (1)
    Use at an assay dependent concentration. Detects a band of approximately 81 kDa (predicted molecular weight: 87 kDa).
    Notes
    WB
    Use at an assay dependent concentration. Detects a band of approximately 81 kDa (predicted molecular weight: 87 kDa).

    Target

    • Function

      Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Expression of TAP1 is down-regulated by human Epstein-Barr virus vIL-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules.
    • Involvement in disease

      Defects in TAP1 are a cause of bare lymphocyte syndrome type 1 (BLS1) [MIM:604571]; also called HLA class I deficiency. BLS1 is a class I antigen deficiency that is not accompanied by particular pathologic manifestations during the first years of life. Systemic infections have not been described. Chronic bacterial infections, often beginning in the first decade of life, are restricted to the respiratory tract.
    • Sequence similarities

      Belongs to the ABC transporter superfamily. ABCB family. MHC peptide exporter (TC 3.A.1.209) subfamily.
      Contains 1 ABC transmembrane type-1 domain.
      Contains 1 ABC transporter domain.
    • Domain

      The peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2.
    • Cellular localization

      Endoplasmic reticulum membrane. The transmembrane segments seem to form a pore in the membrane.
    • Target information above from: UniProt accession Q03518 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6890 Human
      • Omim: 170260 Human
      • SwissProt: Q03518 Human
      • Unigene: 352018 Human
      • Alternative names

        • ABC 17 antibody
        • ABC transporter MHC 1 antibody
        • ABC17 antibody
        • ABCB 2 antibody
        • ABCB2 antibody
        • Antigen peptide transporter 1 antibody
        • APT 1 antibody
        • APT1 antibody
        • ATP binding cassette sub family B (MDR/TAP) member 2 antibody
        • ATP binding cassette sub family B member 2 antibody
        • ATP binding cassette transporter antibody
        • ATP-binding cassette sub-family B member 2 antibody
        • D6S114E antibody
        • FLJ26666 antibody
        • FLJ41500 antibody
        • Peptide supply factor 1 antibody
        • Peptide transporter involved in antigen processing 1 antibody
        • Peptide transporter PSF 1 antibody
        • Peptide transporter PSF1 antibody
        • Peptide transporter TAP 1 antibody
        • Peptide transporter TAP1 antibody
        • PSF 1 antibody
        • PSF-1 antibody
        • PSF1 antibody
        • Really interesting new gene 4 protein antibody
        • RING 4 antibody
        • RING4 antibody
        • TAP 1 antibody
        • TAP1 antibody
        • TAP1*0102N antibody
        • TAP1_HUMAN antibody
        • TAP1N antibody
        • Transporter 1 ATP binding cassette sub family B (MDR/TAP) antibody
        • Transporter 1 ATP binding cassette sub family B antibody
        • Transporter associated with antigen processing antibody
        • Transporter ATP binding cassette major histocompatibility complex 1 antibody
        • Y3 antibody
        see all

      Images

      • Western blot - Anti-TAP1 antibody [53H8] (ab10356)
        Western blot - Anti-TAP1 antibody [53H8] (ab10356)
        All lanes : Anti-TAP1 antibody [53H8] (ab10356) at 5 µg/ml

        Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
        Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

        Predicted band size: 87 kDa
        Observed band size: 81 kDa
        why is the actual band size different from the predicted?
        Additional bands at: 100 kDa (possible non-specific binding)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (1)

      Publishing research using ab10356? Please let us know so that we can cite the reference in this datasheet.

      ab10356 has been referenced in 1 publication.

      • Mo MS  et al. Dopaminergic neurons show increased low-molecular-mass protein 7 activity induced by 6-hydroxydopamine in vitro and in vivo. Transl Neurodegener 7:19 (2018). PubMed: 30128145

      Customer reviews and Q&As

      Show All Reviews Q&A
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      Western blot abreview for Anti-TAP1 antibody [53H8]

      Poor
      Abreviews
      Abreviews
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Cervix)
      Loading amount
      20 µg
      Specification
      Cervix
      Gel Running Conditions
      Reduced Denaturing (12%)
      Blocking step
      AECL (3% w/v) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 18°C
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 25 2008

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