Overview

  • Product name

  • Description

    Rabbit polyclonal to TAP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to internal sequence amino acids 506-644 of Human TAP2.

  • Positive control

    • Human tonsil tissue; A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab130414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.

Target

  • Function

    Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association.
  • Involvement in disease

    Bare lymphocyte syndrome 1
  • Sequence similarities

    Belongs to the ABC transporter superfamily. ABCB family. MHC peptide exporter (TC 3.A.1.209) subfamily.
    Contains 1 ABC transmembrane type-1 domain.
    Contains 1 ABC transporter domain.
  • Domain

    The peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2.
  • Cellular localization

    Endoplasmic reticulum membrane. The transmembrane segments seem to form a pore in the membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ABC transporter, MHC 2 antibody
    • ABC18 antibody
    • ABCB3 antibody
    • Antigen peptide transporter 2 antibody
    • APT2 antibody
    • ATP binding cassette, sub family B (MDR/TAP), member 3 antibody
    • ATP-binding cassette sub-family B member 3 antibody
    • D6S217E antibody
    • Peptide supply factor 2 antibody
    • Peptide transporter involved in antigen processing 2 antibody
    • Peptide transporter PSF2 antibody
    • Peptide transporter TAP2 antibody
    • PSF 2 antibody
    • PSF-2 antibody
    • PSF2 antibody
    • Really interesting new gene 11 protein antibody
    • RING 11 antibody
    • RING11 antibody
    • TAP 2 antibody
    • Tap2 antibody
    • TAP2_HUMAN antibody
    • Transporter 2 ATP binding cassette sub family B antibody
    • Transporter 2, ABC (ATP binding cassette antibody
    • Transporter 2, ATP binding cassette, sub family B (MDR/TAP) antibody
    see all

Images

  • Immunohistochemical analysis of Formalin-fixed, paraffin embedded Human tonsil tissue labelled with TAP2 antibody at 1/500 dilution.
  • Immunofluorescence analysis of PFA/Triton X-100 fixed/permeabilized A431 cells labelled with TAP2 antibody at 4 µg/ml (green).

References

This product has been referenced in:

  • Ween MP  et al. The role of ABC transporters in ovarian cancer progression and chemoresistance. Crit Rev Oncol Hematol 96:220-56 (2015). Read more (PubMed: 26100653) »
See 1 Publication for this product

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