Overview

  • Product name

    Anti-TAPBP-R antibody [OTI1C9]
    See all TAPBP-R primary antibodies
  • Description

    Mouse monoclonal [OTI1C9] to TAPBP-R
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human TAPBP-R. Produced in HEK-293T cells (NP_060479).
    Database link: Q9BX59

  • Positive control

    • WB: HEK-293T transfected with pCMV6-ENTRY TAPBP-R cDNA; HepG2, HeLa, A549, COS-7, Jurkat and MCF7 whole cell lysates. IHC-P: Human pancreas carcinoma and tonsil tissue.
  • General notes

    Clone OTI1C9 (formerly 1C9).

    Previously labelled as Tapasin Related Protein. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Purified from cell culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    OTI1C9
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000. Predicted molecular weight: 50 kDa.

Target

  • Sequence similarities

    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane. Endoplasmic reticulum membrane. Microsome membrane. Mainly found in endoplasmic reticulum but a minority is found on the cell surface.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ10143 antibody
    • TAP binding protein like antibody
    • TAP binding protein related antibody
    • TAP binding protein related protein antibody
    • TAP-binding protein-like antibody
    • TAP-binding protein-related protein antibody
    • Tapasin like antibody
    • Tapasin R antibody
    • Tapasin Related Protein antibody
    • Tapasin-like antibody
    • TAPASIN-R antibody
    • Tapasin-related protein antibody
    • TAPBP L antibody
    • TAPBP R antibody
    • TAPBP-R antibody
    • Tapbpl antibody
    • TAPBPR antibody
    • TPSNR_HUMAN antibody
    see all

Images

  • All lanes : Anti-TAPBP-R antibody [OTI1C9] (ab236419) at 1/2000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK-293T transfected with pCMV6-ENTRY TAPBP-R cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 50 kDa

  • All lanes : Anti-TAPBP-R antibody [OTI1C9] (ab236419) at 1/2000 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 3 : SVT2 cell lysate
    Lane 4 : A549 (human lung carcinoma cell line) cell lysate
    Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) cell lysate
    Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 7 : MDCK (canine kidney cell line) cell lysate
    Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate
    Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 50 kDa

  • Paraffin-embedded human panceas carcinoma tissue stained for TAPBP-R using ab236419 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human tonsil tissue stained for TAPBP-R using ab236419 at 1/100 dilution in immunohistochemical analysis.

References

ab236419 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab236419.
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