Key features and details
- Rabbit polyclonal to TAPP-1
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TAPP-1 antibody
See all TAPP-1 primary antibodies
DescriptionRabbit polyclonal to TAPP-1
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Mouse, Sheep, Chimpanzee, Gorilla
Synthetic peptide within Human TAPP-1 aa 354-404. The exact sequence is proprietary.
Database link: Q9HB21
- IP: Jurkat whole cell lysate (ab7899).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226385 was affinity purified using an epitope specific to TAPP-1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226385 in the following tested applications.
|IP||Use at 2-10 µg/mg of lysate.|
FunctionBinds specifically to phosphatidylinositol 3,4-diphosphate (PtdIns3,4P2), but not to other phosphoinositides. May recruit other proteins to the plasma membrane.
Tissue specificityHighly expressed in skeletal muscle, thymus, pancreas, placenta and lung. Detected at low levels in brain, heart, peripheral blood leukocytes, testis, ovary, spinal cord, thyroid, kidney, liver, small intestine and colon.
Sequence similaritiesContains 2 PH domains.
DomainBinds to membranes enriched in PtdIns3,4P2 via the C-terminal PH domain.
Cellular localizationCytoplasm. Cell membrane. Nucleus. Locates to the plasma membrane after treatments that stimulate the production of PtdIns3,4P2.
- Information by UniProt
- OTTHUMP00000020651 antibody
- PH domain containing family A member 1 antibody
- PH domain-containing family A member 1 antibody
TAPP-1 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226385 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226385 at 1 µg/ml.
Lane 1: ab226385 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
ab226385 has not yet been referenced specifically in any publications.