Overview

  • Product name

  • Description

    Rabbit polyclonal to TAPP-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Sheep, Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide within Human TAPP-1 aa 354-404. The exact sequence is proprietary.
    Database link: Q9HB21

  • Positive control

    • IP: Jurkat whole cell lysate (ab7899).

Properties

Applications

Our Abpromise guarantee covers the use of ab226385 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Binds specifically to phosphatidylinositol 3,4-diphosphate (PtdIns3,4P2), but not to other phosphoinositides. May recruit other proteins to the plasma membrane.
    • Tissue specificity

      Highly expressed in skeletal muscle, thymus, pancreas, placenta and lung. Detected at low levels in brain, heart, peripheral blood leukocytes, testis, ovary, spinal cord, thyroid, kidney, liver, small intestine and colon.
    • Sequence similarities

      Contains 2 PH domains.
    • Domain

      Binds to membranes enriched in PtdIns3,4P2 via the C-terminal PH domain.
    • Cellular localization

      Cytoplasm. Cell membrane. Nucleus. Locates to the plasma membrane after treatments that stimulate the production of PtdIns3,4P2.
    • Information by UniProt
    • Database links

    • Alternative names

      • OTTHUMP00000020651 antibody
      • PH domain containing family A member 1 antibody
      • PH domain-containing family A member 1 antibody
      • PKHA1_HUMAN antibody
      • Pleckstrin homology domain containing A1 antibody
      • Pleckstrin homology domain containing family A member 1 antibody
      • Pleckstrin homology domain containing, family A (phosphoinositide binding specific) member antibody
      • Pleckstrin homology domain-containing family A member 1 antibody
      • PLEKHA1 antibody
      • Tandem PH domain containing protein 1 antibody
      • Tandem PH domain-containing protein 1 antibody
      • TAPP 1 antibody
      • TAPP-1 antibody
      • TAPP1 antibody
      see all

    Images

    • TAPP-1 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226385 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226385 at 1 µg/ml.

      Lane 1: ab226385 IP in Jurkat whole cell lysate.

      Lane 2: Control IgG IP in Jurkat whole cell lysate.

      Detection:  Chemiluminescence with exposure time of 3 minutes.

    References

    ab226385 has not yet been referenced specifically in any publications.

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