Overview

  • Product name

    Anti-TAPP-1 antibody [EPR12061]
    See all TAPP-1 primary antibodies
  • Description

    Rabbit monoclonal [EPR12061] to TAPP-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TAPP-1 aa 300-400 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9HB21

  • Positive control

    • MOLT4, HeLa, Jurkat, A549, Human fetal thymus and Human skeletal muscle lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab174292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 46 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Binds specifically to phosphatidylinositol 3,4-diphosphate (PtdIns3,4P2), but not to other phosphoinositides. May recruit other proteins to the plasma membrane.
    • Tissue specificity

      Highly expressed in skeletal muscle, thymus, pancreas, placenta and lung. Detected at low levels in brain, heart, peripheral blood leukocytes, testis, ovary, spinal cord, thyroid, kidney, liver, small intestine and colon.
    • Sequence similarities

      Contains 2 PH domains.
    • Domain

      Binds to membranes enriched in PtdIns3,4P2 via the C-terminal PH domain.
    • Cellular localization

      Cytoplasm. Cell membrane. Nucleus. Locates to the plasma membrane after treatments that stimulate the production of PtdIns3,4P2.
    • Information by UniProt
    • Database links

    • Alternative names

      • OTTHUMP00000020651 antibody
      • PH domain containing family A member 1 antibody
      • PH domain-containing family A member 1 antibody
      • PKHA1_HUMAN antibody
      • Pleckstrin homology domain containing A1 antibody
      • Pleckstrin homology domain containing family A member 1 antibody
      • Pleckstrin homology domain containing, family A (phosphoinositide binding specific) member antibody
      • Pleckstrin homology domain-containing family A member 1 antibody
      • PLEKHA1 antibody
      • Tandem PH domain containing protein 1 antibody
      • Tandem PH domain-containing protein 1 antibody
      • TAPP 1 antibody
      • TAPP-1 antibody
      • TAPP1 antibody
      see all

    Images

    • All lanes : Anti-TAPP-1 antibody [EPR12061] (ab174292) at 1/1000 dilution

      Lane 1 : MOLT4 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : A549 cell lysate
      Lane 5 : Human fetal thymus cell lysate
      Lane 6 : Human skeletal muscle cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 46 kDa

    • Western blot analysis on immunoprecipitation pellet from (1) MOLT4 cell lysate or (2) 1X PBS (negative control) immunoprecipitated using ab174292 at 1/10 dilution; HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.



      All lanes : Anti-TAPP-1 antibody [EPR12061] (ab174292) at 1/10 dilution

      Lane 1 : MOLT4 cell lysate
      Lane 2 : PBS

      Secondary
      All lanes : HRP-conjugated anti-rabbit IgG

    References

    ab174292 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab174292.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up