Recombinant Anti-TAPP-1 antibody [EPR12061] (ab174292)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12061] to TAPP-1
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TAPP-1 antibody [EPR12061]
See all TAPP-1 primary antibodies -
Description
Rabbit monoclonal [EPR12061] to TAPP-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human TAPP-1 aa 300-400 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9HB21 -
Positive control
- MOLT4, HeLa, Jurkat, A549, Human fetal thymus and Human skeletal muscle lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12061 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab174292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/5000. Predicted molecular weight: 46 kDa.
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IP |
1/10 - 1/100.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 46 kDa. |
IP
1/10 - 1/100. |
Target
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Function
Binds specifically to phosphatidylinositol 3,4-diphosphate (PtdIns3,4P2), but not to other phosphoinositides. May recruit other proteins to the plasma membrane. -
Tissue specificity
Highly expressed in skeletal muscle, thymus, pancreas, placenta and lung. Detected at low levels in brain, heart, peripheral blood leukocytes, testis, ovary, spinal cord, thyroid, kidney, liver, small intestine and colon. -
Sequence similarities
Contains 2 PH domains. -
Domain
Binds to membranes enriched in PtdIns3,4P2 via the C-terminal PH domain. -
Cellular localization
Cytoplasm. Cell membrane. Nucleus. Locates to the plasma membrane after treatments that stimulate the production of PtdIns3,4P2. - Information by UniProt
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Database links
- Entrez Gene: 59338 Human
- Entrez Gene: 101476 Mouse
- Entrez Gene: 361659 Rat
- Omim: 607772 Human
- SwissProt: Q9HB21 Human
- SwissProt: Q8BUL6 Mouse
- Unigene: 643512 Human
- Unigene: 738826 Human
see all -
Alternative names
- OTTHUMP00000020651 antibody
- PH domain containing family A member 1 antibody
- PH domain-containing family A member 1 antibody
see all
Images
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All lanes : Anti-TAPP-1 antibody [EPR12061] (ab174292) at 1/1000 dilution
Lane 1 : MOLT4 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A549 cell lysate
Lane 5 : Human fetal thymus cell lysate
Lane 6 : Human skeletal muscle cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 46 kDa -
Western blot analysis on immunoprecipitation pellet from (1) MOLT4 cell lysate or (2) 1X PBS (negative control) immunoprecipitated using ab174292 at 1/10 dilution; HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
All lanes : Anti-TAPP-1 antibody [EPR12061] (ab174292) at 1/10 dilution
Lane 1 : MOLT4 cell lysate
Lane 2 : PBS
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab174292 has not yet been referenced specifically in any publications.