Overview

  • Product name

  • Description

    Rabbit polyclonal to TARBP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 1571-1621 of Human TARBP1 (NP_005637.3).

  • Positive control

    • HeLa and Jurkat whole cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab129519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 182 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Probable S-adenosyl-L-methionine-dependent methyltransferase which methylates RNA molecules such as tRNAs. In case of infection by HIV-1, it binds to the loop region of TAR RNA, a region also bound by RNA polymerase II. Binding of TARBP1 and RNA polymerase II to HIV-1 TAR RNA is mutually exclusive, suggesting that TARBP1 may function alone or in conjunction with HIV-1 Tat to disengage RNA polymerase II from HIV-1 TAR RNA. May act by methylating HIV-1 TAR RNA.
  • Sequence similarities

    Belongs to the RNA methyltransferase TrmH family.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ30482 antibody
    • Probable methyltransferase TARBP1 antibody
    • TAR (HIV) RNA binding protein 1 antibody
    • TAR antibody
    • TAR RNA loop binding protein antibody
    • TAR RNA-binding protein 1 antibody
    • TAR RNA-binding protein of 185 kDa antibody
    • TARB1_HUMAN antibody
    • TARBP1 antibody
    • TRM3 antibody
    • TRP-185 antibody
    • TRP185 antibody
    see all

Images

  • All lanes : Anti-TARBP1 antibody (ab129519) at 0.4 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 182 kDa


    Exposure time: 3 minutes
  • Detection of TARBP1 by Western blot of immunoprecipitate
    ab129519, at 6 µg/ml, immunoprecipitating TARBP1 in 293T whole cell lysate (1 mg for IP, 20% of IP loaded).
    ab129519 was used at 1 µg/ml for blotting immunoprecipitated TARBP1.
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab129519 has not yet been referenced specifically in any publications.

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