Product nameAnti-TARBP1 antibody
See all TARBP1 primary antibodies
DescriptionRabbit polyclonal to TARBP1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
Synthetic peptide corresponding to a region within C terminal amino acids 1571-1621 of Human TARBP1 (NP_005637.3).
- HeLa and Jurkat whole cell lysates
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab129519 was affinity purified using an epitope specific to TARBP1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab129519 in the following tested applications.
|WB||1/1000 - 1/5000. Predicted molecular weight: 182 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionProbable S-adenosyl-L-methionine-dependent methyltransferase which methylates RNA molecules such as tRNAs. In case of infection by HIV-1, it binds to the loop region of TAR RNA, a region also bound by RNA polymerase II. Binding of TARBP1 and RNA polymerase II to HIV-1 TAR RNA is mutually exclusive, suggesting that TARBP1 may function alone or in conjunction with HIV-1 Tat to disengage RNA polymerase II from HIV-1 TAR RNA. May act by methylating HIV-1 TAR RNA.
Sequence similaritiesBelongs to the RNA methyltransferase TrmH family.
- Information by UniProt
- FLJ30482 antibody
- Probable methyltransferase TARBP1 antibody
- TAR (HIV) RNA binding protein 1 antibody
All lanes : Anti-TARBP1 antibody (ab129519) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 182 kDa
Exposure time: 3 minutes
Detection of TARBP1 by Western blot of immunoprecipitate
ab129519, at 6 µg/ml, immunoprecipitating TARBP1 in 293T whole cell lysate (1 mg for IP, 20% of IP loaded).
ab129519 was used at 1 µg/ml for blotting immunoprecipitated TARBP1.
Detection: Chemiluminescence with exposure time of 30 seconds.
ab129519 has not yet been referenced specifically in any publications.