Recombinant Anti-TARC/CCL17 antibody [EPR15861] (ab195044)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15861] to TARC/CCL17
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TARC/CCL17 antibody [EPR15861]
See all TARC/CCL17 primary antibodies -
Description
Rabbit monoclonal [EPR15861] to TARC/CCL17 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details
Unsuitable for: ICC -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human Hodgkin's lymphoma, non-Hodgkin lymphoma and tonsil tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15861 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab195044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Chemotactic factor for T-lymphocytes but not monocytes or granulocytes. May play a role in T-cell development in thymus and in trafficking and activation of mature T-cells. Binds to CCR4. -
Tissue specificity
Expressed at high levels in thymus and at low levels in the lung, colon and small intestine. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 6361 Human
- Omim: 601520 Human
- SwissProt: Q92583 Human
- Unigene: 546294 Human
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Alternative names
- A-152E5.3 antibody
- A152E53 antibody
- ABCD 2 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling TARC/CCL17 with ab195044 at 1/500 dilution, followed byGoat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 and counter-stained with Hematoxylin.
Inset: Negative control.Note: Cytoplasm staining on human tonsil tissue was observed.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human non-Hodgkin lymphoma tissue labeling TARC/CCL17 with ab195044 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 and counter-stained with Hematoxylin.
Inset: Negative control.Note: Cytoplasm staining on human non-Hodgkin lymphoma tissue was observed.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human Hodgkin lymphoma tissue labeling TARC/CCL17 with ab195044 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 and counter-stained with Hematoxylin.
Inset: Negative control.Note: Cytoplasm staining on humanHodgkin's lymphoma tissue was observed.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab195044 has been referenced in 2 publications.
- Zhang K et al. HBV promotes the recruitment of IL-17 secreting T cells via chemokines CCL22 and CCL17. Liver Int 40:1327-1338 (2020). PubMed: 32187823
- Wang Y et al. HIC1 deletion promotes breast cancer progression by activating tumor cell/fibroblast crosstalk. J Clin Invest 128:5235-5250 (2018). PubMed: 30204129