Recombinant
RabMAb

Recombinant Anti-TARC/CCL17 antibody [EPR15861] - BSA and Azide free (ab232659)

Overview

  • Product name

    Anti-TARC/CCL17 antibody [EPR15861] - BSA and Azide free
    See all TARC/CCL17 primary antibodies
  • Description

    Rabbit monoclonal [EPR15861] to TARC/CCL17 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human TARC/CCL17 aa 1-100. The exact sequence is proprietary. The immunogen corresponds to the mature form of TARC/CCL17.
    Database link: Q92583

  • Positive control

    • IHC-P: Human Hodgkin's lymphoma tissue.
  • General notes

    ab232659 is the carrier-free version of ab195044 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab232659 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as TARC

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15861
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab232659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Chemotactic factor for T-lymphocytes but not monocytes or granulocytes. May play a role in T-cell development in thymus and in trafficking and activation of mature T-cells. Binds to CCR4.
  • Tissue specificity

    Expressed at high levels in thymus and at low levels in the lung, colon and small intestine.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • A-152E5.3 antibody
    • A152E53 antibody
    • ABCD 2 antibody
    • ABCD2 antibody
    • C-C motif chemokine 17 antibody
    • CC chemokine TARC antibody
    • CCL17 antibody
    • CCL17_HUMAN antibody
    • Chemokine CC Motif Ligand 17 antibody
    • MGC138271 antibody
    • MGC138273 antibody
    • SCYA17 antibody
    • Small Inducible Cytokine A17 antibody
    • Small Inducible Cytokine A17 Precursor antibody
    • Small Inducible Cytokine Subfamily A (Cys Cys) antibody
    • Small Inducible Cytokine Subfamily A (Cys Cys) Member 17 antibody
    • Small-inducible cytokine A17 antibody
    • T Cell Directed CC Chemokine antibody
    • Thymus and activation regulated chemokine antibody
    • Thymus and activation-regulated chemokine antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling TARC/CCL17 with ab195044 at 1/500 dilution, followed byGoat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 and counter-stained with Hematoxylin.
    Inset: Negative control.

    Note: Cytoplasm staining on human tonsil tissue was observed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195044).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human non-Hodgkin lymphoma tissue labeling TARC/CCL17 with ab195044 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 and counter-stained with Hematoxylin.
    Inset: Negative control.

    Note: Cytoplasm staining on human non-Hodgkin lymphoma tissue was observed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195044).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human Hodgkin lymphoma tissue labeling TARC/CCL17 with ab195044 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 and counter-stained with Hematoxylin. Cytoplasm staining on humanHodgkin's lymphoma tissue was observed.
    Inset: Negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab195044).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab232659 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab232659.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up