Anti-TARSL2 antibody - C-terminal (ab175945)

Overview

  • Product name
    Anti-TARSL2 antibody - C-terminal
    See all TARSL2 primary antibodies
  • Description
    Rabbit polyclonal to TARSL2 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human TARSL2 aa 644-674 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: A2RTX5

  • Positive control
    • Mouse brain tissue; 293 cell line lysates; 293 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab175945 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 93 kDa.

Target

  • Relevance
    TARSL2 belongs to the class-II aminoacyl-tRNA synthetase family and it has two isoforms.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • FLJ25005 antibody
    • Threonine tRNA ligase antibody
    • threonyl tRNA synthetase like 2 antibody
    • Threonyl-tRNA synthetase-like protein 2 antibody
    • ThrRS antibody
    see all

Images

  • Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Mouse brain tissue labeling TARSL2 with ab175945 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.

  • Anti-TARSL2 antibody - C-terminal (ab175945) at 1/1000 dilution + 293 cell line lysates at 35 µg

    Predicted band size: 93 kDa

  • Flow Cytometric analysis of 293 cells labeling TARSL2 with ab175945 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab175945 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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