Overview

  • Product name

    Anti-Tartrate Resistant Acid Phosphatase antibody
    See all Tartrate Resistant Acid Phosphatase primary antibodies
  • Description

    Rabbit polyclonal to Tartrate Resistant Acid Phosphatase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Pig
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 28 - 315 of Human Tartrate Resistant Acid Phosphatase

  • Positive control

    • A431, MOLT4 and NIH3T3 cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab96372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 37 kDa.

Target

  • Function

    Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias.
  • Sequence similarities

    Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.
  • Cellular localization

    Lysosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • Type 5 acid phosphatase antibody
    • Acid phosphatase 5 tartrate resistant antibody
    • Acid phosphatase 5, tartrate resistant antibody
    • ACP5 antibody
    • EC 3.1.3.2 antibody
    • MGC117378 antibody
    • PPA5_HUMAN antibody
    • serum band 5 tartrate-resistant acid phosphatase antibody
    • SPENCDI antibody
    • T5ap antibody
    • Tartrate resistant acid ATPase antibody
    • Tartrate resistant acid phosphatase type 5 antibody
    • Tartrate resistant acid phosphatase type 5 precursor antibody
    • Tartrate-resistant acid ATPase antibody
    • Tartrate-resistant acid phosphatase type 5 antibody
    • TR AP antibody
    • TR-AP antibody
    • TRACP 5 antibody
    • TRAP antibody
    • TrATPase antibody
    • Type 5 acid phosphatase antibody
    see all

Images

  • All lanes : Anti-Tartrate Resistant Acid Phosphatase antibody (ab96372) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : MOLT4 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 37 kDa
    Additional bands at: 43 kDa. We are unsure as to the identity of these extra bands.



    12% SDS PAGE
  • Anti-Tartrate Resistant Acid Phosphatase antibody (ab96372) at 1/1000 dilution + NIH3T3 whole cell lysate at 30 µg

    Predicted band size: 37 kDa



    10% SDS PAGE

References

This product has been referenced in:

  • Wu C  et al. Wenshen Zhuanggu formula mitigates breast cancer bone metastasis through the signaling crosstalk among the Jagged1/Notch, TGF-ß and IL-6 signaling pathways. J Ethnopharmacol 232:145-154 (2019). Read more (PubMed: 30576770) »
  • Mi B  et al. Strontium-loaded titania nanotube arrays repress osteoclast differentiation through multiple signalling pathways: In vitro and in vivo studies. Sci Rep 7:2328 (2017). Read more (PubMed: 28539667) »
See all 5 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

The Immunogen sequence of ab96372 is quite homologous with rat so I am assuming this antibody would cross react with rat TRAP protein. I am happy to provide discount code if the customer is OK to buy this product.

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Answer

Oui, n'hésitez pas à me contacter de nouveau si vous avez plus d'informations concernant la différence entre la forme 5a et 5b, je serai ravi de chercher l'anticorps qui convient à vos travaux.

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Question
Answer

Merci de nous avoir contactés.

Les quatre anticorps anti-TRAP (type 5)que nous avons sont :
ab49507
ab96372
ab75452
ab14054
Ils sont tous dirigés vers la protéine dont la référence UniProt est P13686(http://www.uniprot.org/uniprot/P13686)

Je n'ai pas trouvé d'information sur la différence entre la séquence en acides aminés de la forme 5a et celle de la forme 5b.
Si vous disposez de cette information, j'essaierai alors de trouver un produit qui convienne à vos travaux.

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Answer

Thank you for your enquiry.

I am sorry to confirm that ab96372 has not yet been tested in IHC-P. I am pleased to hear the customer would like to make use of our testing discount program.

I can provide the following code for the customer to test ab96372 in IHC-P:

DISCOUNT CODE: 41623
Expiration date: 27th July 2012

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forWB and include this code in the Additional Comments section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

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