Recombinant
RabMAb

Recombinant Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406)

Overview

  • Product name

    Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556]
    See all Tartrate Resistant Acid Phosphatase primary antibodies
  • Description

    Rabbit monoclonal [EPR15556] to Tartrate Resistant Acid Phosphatase
  • Host species

    Rabbit
  • Specificity

    This antibody gave negative results in IHC-P in both mouse and rat species. Both species were also tested in WB with positive results. Please do not hesitate to contact our Scientific Support if you have any questions.
  • Tested applications

    Suitable for: IP, ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Tartrate Resistant Acid Phosphatase aa 150-300. The exact sequence is proprietary.
    Database link: P13686

  • Positive control

    • A431, MDA-MB435, C6, Raw 264.7, PC-12 and NIH/3T3 cell lysates; Human tonsil and fetal kidney lysates; Human tonsil tissue; MDA-MB435 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab191406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/50.
ICC/IF 1/100.
WB 1/1000 - 1/10000. Detects a band of approximately 35, 42 kDa (predicted molecular weight: 37 kDa).

Per the literature, a novel previously unreported 42 kDa variant of the Tartrate Resistant Acid Phosphatase 5a isoform was identified. So the 42 kDa band should be the Tartrate Resistant Acid Phosphatase 5a isoform.

IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias.
  • Sequence similarities

    Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.
  • Cellular localization

    Lysosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • Type 5 acid phosphatase antibody
    • Acid phosphatase 5 tartrate resistant antibody
    • Acid phosphatase 5, tartrate resistant antibody
    • ACP5 antibody
    • EC 3.1.3.2 antibody
    • MGC117378 antibody
    • PPA5_HUMAN antibody
    • serum band 5 tartrate-resistant acid phosphatase antibody
    • SPENCDI antibody
    • T5ap antibody
    • Tartrate resistant acid ATPase antibody
    • Tartrate resistant acid phosphatase type 5 antibody
    • Tartrate resistant acid phosphatase type 5 precursor antibody
    • Tartrate-resistant acid ATPase antibody
    • Tartrate-resistant acid phosphatase type 5 antibody
    • TR AP antibody
    • TR-AP antibody
    • TRACP 5 antibody
    • TRAP antibody
    • TrATPase antibody
    • Type 5 acid phosphatase antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Tartrate Resistant Acid Phosphatase with ab191406 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    In the negative control PBS was used instead of primary antibody.

  • All lanes : Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406) at 1/10000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : MDA-MB435 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM /TBST.

  • All lanes : Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406) at 1/1000 dilution

    Lane 1 : Human tonsil lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 35,42 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM /TBST.

     

    Per the literature, a novel previously unreported 42 kDa variant of the TRAP 5a isoform was identified. So the 42 kDa band should be the TRAP 5a isoform.

  • All lanes : Anti-Tartrate Resistant Acid Phosphatase antibody [EPR15556] (ab191406) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : Raw264.7 cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : NIH 3T3/cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 37 kDa
    Observed band size: 42 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM /TBST.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized MDA-MB435 cells labeling Tartrate Resistant Acid Phosphatase with ab191406 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor®488) secondary antibody (ab150077) at 1/200 dilution. Nuclear counter stain DAPI (blue).

    The two negative controls used anti-TRAP 5 primary antibody at 1/100 dilution, followed by Goat anti-mouse IgG (Alexa Fluor®594) secondary antibody at 1/400 dilution.

  • Western blot analysis of TRAP 5 in MDA-MB435 cell lysate immunoprecipitated using ab191406 at 1/50 dilution (Lane 1). Lane 2: PBS instead of MDA-MB-435 lysates.

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution.

References

This product has been referenced in:

  • Bian ZQ  et al. Overexpressed ACP5 has prognostic value in colorectal cancer and promotes cell proliferation and tumorigenesis via FAK/PI3K/AKT signaling pathway. Am J Cancer Res 9:22-35 (2019). Read more (PubMed: 30755809) »
  • Gao Y & Ge W The histone methyltransferase DOT1L inhibits osteoclastogenesis and protects against osteoporosis. Cell Death Dis 9:33 (2018). Read more (PubMed: 29348610) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (vertebrae)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH 9.0
Permeabilization
No
Specification
vertebrae
Blocking step
(agent) for 10 minute(s) · Concentration: 100% · Temperature: 21°C
Fixative
10%NBF

Zhirui Jiang

Verified customer

Submitted Mar 13 2019

Application
IHC - Wholemount
Sample
Mouse Tissue (Whole Tibia)
Specification
Whole Tibia

Abcam user community

Verified customer

Submitted Oct 24 2018

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