Overview

  • Product name

    Anti-Tat-SF1 antibody [EPR9104(B)]
    See all Tat-SF1 primary antibodies
  • Description

    Rabbit monoclonal [EPR9104(B)] to Tat-SF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Tat-SF1 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Jurkat and HeLa cell lysates; Human colon and uterus tissue; HeLa cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    Previously labelled as HIV TAT specific factor 1.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab140654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 86 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP 1/10 - 1/100.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Relevance

      HIV TAT specific factor(a.k.a. HTATSF1, Tat-SF1 or HTSF1) is an 86 kDa general transcription factor that plays a role in the process of transcription elongation. However, in HIV-infected cells, this factor is up-regulated by HIV Nef and gp120 and acts as a co-factor for the Tat-enhanced transcription of the HIV virus.
    • Cellular localization

      Nuclear
    • Database links

    • Alternative names

      • Cofactor required for Tat activation of HIV 1 transcription antibody
      • dJ196E23.2 antibody
      • HIV Tat specific factor 1 antibody
      • HTATSF1 antibody
      • HTSF-1 antibody
      • HTSF1 antibody
      • TAT SF1 antibody
      • Tat-SF1 antibody
      see all

    Images

    • All lanes : Anti-Tat-SF1 antibody [EPR9104(B)] (ab140654) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 86 kDa
      Observed band size: 140 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling Tat-SF1 with ab140654 at 1/250 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human uterus tissue labelling Tat-SF1 with ab140654 at 1/250 dilution.

    • Immunofluorescent analysis of HeLa cells labelling Tat-SF1 with ab140654 at 1/250 dilution.

    References

    ab140654 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab140654.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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