Product nameAnti-Tat-SF1 antibody [EPR9104(B)]
See all Tat-SF1 primary antibodies
DescriptionRabbit monoclonal [EPR9104(B)] to Tat-SF1
Tested applicationsSuitable for: WB, IHC-P, IP, ICC/IFmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human Tat-SF1 aa 1-100. The exact sequence is proprietary.
- Jurkat and HeLa cell lysates; Human colon and uterus tissue; HeLa cells.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Previously labelled as HIV TAT specific factor 1.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab140654 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 86 kDa).|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IP||1/10 - 1/100.|
|ICC/IF||1/250 - 1/500.|
RelevanceHIV TAT specific factor(a.k.a. HTATSF1, Tat-SF1 or HTSF1) is an 86 kDa general transcription factor that plays a role in the process of transcription elongation. However, in HIV-infected cells, this factor is up-regulated by HIV Nef and gp120 and acts as a co-factor for the Tat-enhanced transcription of the HIV virus.
- Cofactor required for Tat activation of HIV 1 transcription antibody
- dJ196E23.2 antibody
- HIV Tat specific factor 1 antibody
All lanes : Anti-Tat-SF1 antibody [EPR9104(B)] (ab140654) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 86 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling Tat-SF1 with ab140654 at 1/250 dilution.
Immunohistochemical analysis of paraffin-embedded Human uterus tissue labelling Tat-SF1 with ab140654 at 1/250 dilution.
Immunofluorescent analysis of HeLa cells labelling Tat-SF1 with ab140654 at 1/250 dilution.
ab140654 has not yet been referenced specifically in any publications.