Product nameAnti-Tat-SF1 antibody [EPR9105(B)]
See all Tat-SF1 primary antibodies
DescriptionRabbit monoclonal [EPR9105(B)] to Tat-SF1
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Rat, Human
Synthetic peptide within Human Tat-SF1 aa 350-450. The exact sequence is proprietary.
- HeLa and Jurkat cell lysates. Human colon tissue. HepG2 cells.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Previously labelled as HIV TAT specific factor 1.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C.
Dissociation constant (KD)KD = 8.00 x 10 -12 M Learn more about KD
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134921 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 86 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/250 - 1/500.|
|IP||1/10 - 1/100.|
RelevanceHIV TAT specific factor(a.k.a. HTATSF1, Tat-SF1 or HTSF1) is an 86 kDa general transcription factor that plays a role in the process of transcription elongation. However, in HIV-infected cells, this factor is up-regulated by HIV Nef and gp120 and acts as a co-factor for the Tat-enhanced transcription of the HIV virus.
- Cofactor required for Tat activation of HIV 1 transcription antibody
- dJ196E23.2 antibody
- HIV Tat specific factor 1 antibody
All lanes : Anti-Tat-SF1 antibody [EPR9105(B)] (ab134921) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 86 kDa
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling Tat-SF1 with ab134921 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Tat-SF1 with ab134921 at 1/100 dilution.
Immunofluorescence analysis of HepG2 cells staining Tat-SF1 with ab134921 at 1/250 dilution.
Immunohistochemical analysis using ab134921 showing positive staining in Normal testis tissue.
Immunohistochemical analysis using ab134921 showing positive staining in Breast carcinoma tissue.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab134921 has not yet been referenced specifically in any publications.