Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818)
Key features and details
- Mouse monoclonal [1TBP18] to TATA binding protein TBP - ChIP Grade
- Suitable for: ICC, IHC-Fr, IP, ICC/IF, IHC-P, ChIP/Chip, EMSA, ELISA, Flow Cyt, WB, ChIP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade
See all TATA binding protein TBP primary antibodies -
Description
Mouse monoclonal [1TBP18] to TATA binding protein TBP - ChIP Grade -
Host species
Mouse -
Tested applications
Suitable for: ICC, IHC-Fr, IP, ICC/IF, IHC-P, ChIP/Chip, EMSA, ELISA, Flow Cyt, WB, ChIPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Does not react with: Saccharomyces cerevisiae, Xenopus laevis, Drosophila melanogaster, Silk worm -
Immunogen
Synthetic peptide corresponding to Human TATA binding protein TBP.
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Epitope
Within amino acid residues 1-20 of human, mouse and rat TBP. The specific epitope for this antibody is accessible when the C-terminal domain of TBP is removed or when TBP is complexed with DNA. -
General notes
This product was changed from ascites to tissue culture supernatant on 19/12/2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
1TBP18 -
Isotype
IgG1 -
Research areas
- Epigenetics and Nuclear Signaling
- Transcription
- Polymerase associated factors
- Pol II Transcription
- TFIIB
- Epigenetics and Nuclear Signaling
- Transcription
- Polymerase associated factors
- Pol II Transcription
- TFIID
Associated products
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ChIP Related Products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab818 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC | (1) |
Use at an assay dependent concentration.
|
IHC-Fr | (1) |
Use at an assay dependent concentration.
|
IP | (2) |
Use at an assay dependent concentration.
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ICC/IF | (4) |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use at an assay dependent concentration.
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ChIP/Chip | (1) |
Use at an assay dependent concentration.
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EMSA |
Use at an assay dependent concentration.
|
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ELISA | (2) |
Use at an assay dependent concentration.
We recommend Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab6729) secondary antibody. |
Flow Cyt | ||
WB | (24) |
Use at an assay dependent concentration. Predicted molecular weight: 38 kDa. PubMed: 23767827
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ChIP | (2) |
Use 5-10 µg for 25 µg of chromatin.
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Notes |
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ICC
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
ChIP/Chip
Use at an assay dependent concentration. |
EMSA
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. We recommend Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab6729) secondary antibody. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 38 kDa. PubMed: 23767827 |
ChIP
Use 5-10 µg for 25 µg of chromatin. |
Target
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Function
General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. -
Tissue specificity
Widely expressed, with levels highest in the testis and ovary. -
Involvement in disease
Defects in TBP are the cause of spinocerebellar ataxia type 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease. -
Sequence similarities
Belongs to the TBP family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6908 Human
- Entrez Gene: 21374 Mouse
- Entrez Gene: 117526 Rat
- Omim: 600075 Human
- SwissProt: P20226 Human
- SwissProt: P29037 Mouse
- Unigene: 590872 Human
- Unigene: 244820 Mouse
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Alternative names
- GTF2D antibody
- GTF2D1 antibody
- HDL4 antibody
see all
Images
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Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 8 µg of ab818 (blue), and 20 µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The last wash was performed with final wash buffer containing 250 mM NaCl. The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes are located in the core promoter region of the genes.
This image was generated using the ascites version of the product.
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Overlay histogram showing HeLa cells stained with ab818 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab818, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879) secondary antibody at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (257)
ab818 has been referenced in 257 publications.
- Tremblay MG et al. Ribosomal DNA promoter recognition is determined in vivo by cooperation between UBTF1 and SL1 and is compromised in the UBTF-E210K neuroregression syndrome. PLoS Genet 18:e1009644 (2022). PubMed: 35139074
- Jiang Z et al. KLF15 cistromes reveal a hepatocyte pathway governing plasma corticosteroid transport and systemic inflammation. Sci Adv 8:eabj2917 (2022). PubMed: 35263131
- Wang Z et al. Prediction of histone post-translational modification patterns based on nascent transcription data. Nat Genet 54:295-305 (2022). PubMed: 35273399
- Franklin R et al. Regulation of chromatin accessibility by the histone chaperone CAF-1 sustains lineage fidelity. Nat Commun 13:2350 (2022). PubMed: 35487911
- Dimitrova E et al. Distinct roles for CKM-Mediator in controlling Polycomb-dependent chromosomal interactions and priming genes for induction. Nat Struct Mol Biol 29:1000-1010 (2022). PubMed: 36220895