Western blot abreview for Anti-TATA binding protein TBP [1TBP18] antibody - ChIP Grade

Excellent
Abreviews
Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Rat Cell lysate - nuclear (airway epithelial cell)
Specification
airway epithelial cell
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk

Secondary antibody

Name
Dilution
1/2000

Detection

Detection method
ECL
Bands
Specific: 40 kDa Non-specific: 30, 55 kDa
Exposure
5 minute(s) and 0 second(s)
Negative control
Rat cytoplasmic lysate
Positive control
Rat nuclear lysate

Additional data

Additional Notes
Lane 1 = magic mark Lane 2= Empty Lanes 3-8 = rat airway epithelial cell nuclear lysate Lane 9 = rat airway epithelial cell cytoplasmic lysate

Joseph Cichocki

Verified customer

Submitted Mar 07 2014

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