Anti-TATA binding protein TBP antibody [58C9] (ab61411)


  • Product name
    Anti-TATA binding protein TBP antibody [58C9]
    See all TATA binding protein TBP primary antibodies
  • Description
    Mouse monoclonal [58C9] to TATA binding protein TBP
  • Host species
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human, Saccharomyces cerevisiae, Drosophila melanogaster, Corn
  • Immunogen

    Drosophila TFIID complex.

  • Positive control
    • Nuclear extracts of D melanogaster cells.



Our Abpromise guarantee covers the use of ab61411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 38 kDa).
IP Use at an assay dependent dilution.


  • Function
    General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.
  • Tissue specificity
    Widely expressed, with levels highest in the testis and ovary.
  • Involvement in disease
    Defects in TBP are the cause of spinocerebellar ataxia type 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.
  • Sequence similarities
    Belongs to the TBP family.
  • Cellular localization
  • Information by UniProt
  • Database links
    • Alternative names
      • GTF2D antibody
      • GTF2D1 antibody
      • HDL4 antibody
      • MGC117320 antibody
      • MGC126054 antibody
      • MGC126055 antibody
      • SCA17 antibody
      • TATA binding factor antibody
      • TATA box factor antibody
      • TATA sequence binding protein antibody
      • TATA sequence-binding protein antibody
      • TATA-binding factor antibody
      • TATA-box binding protein N-terminal domain antibody
      • TATA-box factor antibody
      • TATA-box-binding protein antibody
      • TBP antibody
      • TBP_HUMAN antibody
      • TFIID antibody
      • Transcription initiation factor TFIID TBP subunit antibody
      see all


    • Lane 1 : Anti-TATA binding protein TBP antibody [58C9] (ab61411) at 2 µg/ml
      Lane 2 : Anti-TATA binding protein TBP antibody [58C9] (ab61411) at 1 µg/ml
      Lane 3 : Anti-TATA binding protein TBP antibody [58C9] (ab61411) at 0.5 µg/ml
      Lane 4 : Anti-TATA binding protein TBP antibody [58C9] (ab61411) at 0.2 µg/ml
      Lane 5 : Anti-TATA binding protein TBP antibody [58C9] (ab61411) (Negative control )

      All lanes : Nuclear cell extract of D.Mel cells

      Lysates/proteins at 20 µg/ml per lane.

      All lanes : Goat Anti-Mouse – Peroxidase conjugate

      Developed using the ECL technique.

      Predicted band size: 38 kDa
      Observed band size: 39 kDa (why is the actual band size different from the predicted?)


    This product has been referenced in:
    • Hoffman EA  et al. An Improved Method for Measuring Chromatin-binding Dynamics Using Time-dependent Formaldehyde Crosslinking. Bio Protoc 8:N/A (2018). Read more (PubMed: 29682595) »
    • Young CP  et al. Distinct histone methylation and transcription profiles are established during the development of cellular quiescence in yeast. BMC Genomics 18:107 (2017). WB ; Saccharomyces cerevisiae . Read more (PubMed: 28122508) »

    See all 10 Publications for this product

    Customer reviews and Q&As

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    I am sorry to confirm that our source has not determined whether this particular antibody is kappa or light chain.

    I am sorry we do not have the information you require on this occasion. If you have any...

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    I am pleased to let you know I now have further information regarding ab24685 and ab61411

    Our datasheet says that this reacts with AA 41-57 of the human sequence. This has11% alignment with...

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    Thank you for contacting us and for your interest in our products.

    As far as we are aware, these TATA binding protein antibodies (ab61411, ab24685, ab28175) have never been tested with samples from Methanococcoides burtonii. All tested speci...

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