Anti-TATA binding protein TBP antibody (ab28175)
Key features and details
- Rabbit polyclonal to TATA binding protein TBP
- Suitable for: ChIP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-TATA binding protein TBP antibody
See all TATA binding protein TBP primary antibodies -
Description
Rabbit polyclonal to TATA binding protein TBP -
Host species
Rabbit -
Specificity
Ab28175 recognises TATA binding protein TBP. -
Tested Applications & Species
See all applications and species dataApplication Species ChIP Human
-
Immunogen
Recombinant full length TATA binding protein TBP (Human).
-
General notes
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.9
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
ChIP Related Products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ChIP |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| ChIP | (2) |
Use 5-10 µg for 25 µg of chromatin.
|
| Notes |
|---|
|
ChIP
Use 5-10 µg for 25 µg of chromatin. |
Target
-
Function
General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. -
Tissue specificity
Widely expressed, with levels highest in the testis and ovary. -
Involvement in disease
Defects in TBP are the cause of spinocerebellar ataxia type 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease. -
Sequence similarities
Belongs to the TBP family. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 6908 Human
- Omim: 600075 Human
- SwissProt: P20226 Human
- Unigene: 1100 Human
- Unigene: 590872 Human
-
Alternative names
- GTF2D antibody
- GTF2D1 antibody
- HDL4 antibody
see all
Images
-
ChIP - Anti-TATA binding protein TBP antibody (ab28175)This image is courtesy of an anonymous Abreview
ChIP analysis using ab28175 binding TATA binding protein TBP in mouse liver nuclear tissue lysate. Cells were cross-linked for 10 minutes with 1% paraformaldehyde. Samples were incubated with primary antibody for 16 hours at 4°C in TE pH8, 150mM NaCl, Trition 1%, SDS 0,1%. Protein binding was detected using real-time PCR.
Negative Control: beads. -
ChIP - Anti-TATA binding protein TBP antibody (ab28175)Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 8µg of ab28175 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes are located either in the core promoter of the gene (TATA) or in the open reading frame (ORF).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (13)
ab28175 has been referenced in 13 publications.
- Le SN et al. The TAFs of TFIID Bind and Rearrange the Topology of the TATA-Less RPS5 Promoter. Int J Mol Sci 20:N/A (2019). PubMed: 31277458
- Sachini N et al. Promyelocytic leukemia protein (PML) controls breast cancer cell proliferation by modulating Forkhead transcription factors. Mol Oncol 13:1369-1387 (2019). PubMed: 30927552
- Gilbertson S et al. Changes in mRNA abundance drive shuttling of RNA binding proteins, linking cytoplasmic RNA degradation to transcription. Elife 7:N/A (2018). PubMed: 30281021
- Wu B et al. Aldehyde dehydrogenase 2 activation in aged heart improves the autophagy by reducing the carbonyl modification on SIRT1. Oncotarget 7:2175-88 (2016). Mouse . PubMed: 26741505
- Martianov I et al. TRF2 is recruited to the pre-initiation complex as a testis-specific subunit of TFIIA/ALF to promote haploid cell gene expression. Sci Rep 6:32069 (2016). Mouse . PubMed: 27576952