Overview

  • Product name
    Anti-TATA binding protein TBP antibody
    See all TATA binding protein TBP primary antibodies
  • Description
    Rabbit polyclonal to TATA binding protein TBP
  • Host species
    Rabbit
  • Specificity
    Ab28175 recognises TATA binding protein TBP.
  • Tested applications
    Suitable for: WB, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length TATA binding protein TBP (Human).

  • Positive control
    • HeLa nuclear extract.
  • General notes

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab28175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Predicted molecular weight: 38 kDa.
ChIP Use 5-10 µg for 25 µg of chromatin.

Target

  • Function
    General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.
  • Tissue specificity
    Widely expressed, with levels highest in the testis and ovary.
  • Involvement in disease
    Defects in TBP are the cause of spinocerebellar ataxia type 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.
  • Sequence similarities
    Belongs to the TBP family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • GTF2D antibody
    • GTF2D1 antibody
    • HDL4 antibody
    • MGC117320 antibody
    • MGC126054 antibody
    • MGC126055 antibody
    • SCA17 antibody
    • TATA binding factor antibody
    • TATA box factor antibody
    • TATA sequence binding protein antibody
    • TATA sequence-binding protein antibody
    • TATA-binding factor antibody
    • TATA-box binding protein N-terminal domain antibody
    • TATA-box factor antibody
    • TATA-box-binding protein antibody
    • TBP antibody
    • TBP_HUMAN antibody
    • TFIID antibody
    • Transcription initiation factor TFIID TBP subunit antibody
    see all

Images

  • ChIP analysis using ab28175 binding TATA binding protein TBP in mouse liver nuclear tissue lysate. Cells were cross-linked for 10 minutes with 1% paraformaldehyde. Samples were incubated with primary antibody for 16 hours at 4°C in TE pH8, 150mM NaCl, Trition 1%, SDS 0,1%. Protein binding was detected using real-time PCR.
    Negative Control: beads.

    See Abreview

  • Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 8µg of  ab28175 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes are located either in the core promoter of the gene (TATA) or in the open reading frame (ORF).
        

References

This product has been referenced in:
  • Wu B  et al. Aldehyde dehydrogenase 2 activation in aged heart improves the autophagy by reducing the carbonyl modification on SIRT1. Oncotarget 7:2175-88 (2016). Mouse . Read more (PubMed: 26741505) »
  • Martianov I  et al. TRF2 is recruited to the pre-initiation complex as a testis-specific subunit of TFIIA/ALF to promote haploid cell gene expression. Sci Rep 6:32069 (2016). Mouse . Read more (PubMed: 27576952) »
See all 10 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ChIP
Detection step
Real-time PCR
Sample
Mouse Tissue lysate - nuclear (Liver)
Specification
Liver
Negative control
beads
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: PFA 1%

Abcam user community

Verified customer

Submitted Nov 12 2012

Answer

Thank you for your message.

I am pleased to let you know I now have further information regarding ab24685 and ab61411

ab24685
Our datasheet says that this reacts with AA 41-57 of the human sequence. This has11% alignment with the Methanococcoides burtonii sequence you provided.I am sorry that we would therefore not expect this antibody to detect.

ab61411
The immunogen used to raisethis antibodywas Drosophila TFIID complex (Gene ID: 37476). This has 27% alignment with the Methanococcoides burtonii sequence you provided.I am sorry that we woudl therefore not expect this antibody to detect.

Please note we cannot guarantee that any of these three antibodies would work in the Methanococcoides burtonii. As a gesture of goodwill, Iam able to provide a 5% discount for you, but I am sorry I would not expect positive results.

In order to receive the discount, please provide the following discount code when placing the order: 5PC-OFF-T7T62.

I hope this will be helpful. If you have any further questions, please do not hesitate to contactus.

Read More

Answer

Thank you for contacting us and for your interest in our products.

As far as we are aware, these TATA binding protein antibodies (ab61411, ab24685, ab28175) have never been tested with samples from Methanococcoides burtonii. All tested species cross-reactivity information is stated on our datasheets, and these are updated as soon as any new information is brought to our attention.

As you requested this information as urgent, I can confirm the following information that I have collected so far:

ab28175: I can confirm this is tested and guaranteed in WB and ChIP. All tested and guaranteed applications are listed on the online datasheet, and these are updated as soon as we receive any new information. The immunogen, as stated on the datasheet, is recombinant full length TATA binding protein TBP (Human). I have checked the alignment of the human protein with the sequence you have kindly provided, I am sorry this is regrettably low at 27%. We would therefore not expect this antibody to detect the Methanococcoides burtoniiprotein.

ab24685: I am sorry this is tested and guaranteed in WB and Gel supershift assays only, It has not yet been tested in ChIP. I am currently contacting our source to obtain more specific details regarding the immunogen so I can check the alignment with the Methanococcoides burtoniisequence for you.

ab61411: I am sorry this is tested and guaranteed in WB andIP only, It has not yet been tested in ChIP. I am currently contacting our source to obtain more specific details regarding the immunogen so I can check the alignment with the Methanococcoides burtoniiseqeuence for you.

I will get back to you as soon as I have further information regarding ab24685 and ab61411.Thank you for your patience. If you require any further information, please do not hesitate to let me know.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ChIP
Sample
Mouse Cell lysate - whole cell (3T3 cells)
Specification
3T3 cells
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Detection step
Real-time PCR
Negative control
anti-CD30

Abcam user community

Verified customer

Submitted Feb 11 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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