Anti-TATA binding protein TBP antibody (ab28175)
- Datasheet
- References (13)
- Protocols
Overview
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Product name
Anti-TATA binding protein TBP antibody
See all TATA binding protein TBP primary antibodies -
Description
Rabbit polyclonal to TATA binding protein TBP -
Host species
Rabbit -
Specificity
Ab28175 recognises TATA binding protein TBP. -
Tested applications
Suitable for: WB, ChIPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length TATA binding protein TBP (Human).
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Positive control
- HeLa nuclear extract.
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General notes
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab28175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000 - 1/10000. Predicted molecular weight: 38 kDa. | |
| ChIP | Use 5-10 µg for 25 µg of chromatin. |
Target
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Function
General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. -
Tissue specificity
Widely expressed, with levels highest in the testis and ovary. -
Involvement in disease
Defects in TBP are the cause of spinocerebellar ataxia type 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease. -
Sequence similarities
Belongs to the TBP family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6908 Human
- Entrez Gene: 21374 Mouse
- Omim: 600075 Human
- SwissProt: P20226 Human
- SwissProt: P29037 Mouse
- Unigene: 1100 Human
- Unigene: 590872 Human
- Unigene: 244820 Mouse
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Alternative names
- GTF2D antibody
- GTF2D1 antibody
- HDL4 antibody
see all
Images
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ChIP - Anti-TATA binding protein TBP antibody (ab28175)This image is courtesy of an anonymous AbreviewChIP analysis using ab28175 binding TATA binding protein TBP in mouse liver nuclear tissue lysate. Cells were cross-linked for 10 minutes with 1% paraformaldehyde. Samples were incubated with primary antibody for 16 hours at 4°C in TE pH8, 150mM NaCl, Trition 1%, SDS 0,1%. Protein binding was detected using real-time PCR.
Negative Control: beads. -
ChIP - Anti-TATA binding protein TBP antibody (ab28175)Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 8µg of ab28175 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes are located either in the core promoter of the gene (TATA) or in the open reading frame (ORF).
Datasheets and documents
References
This product has been referenced in:
- Le SN et al. The TAFs of TFIID Bind and Rearrange the Topology of the TATA-Less RPS5 Promoter. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 31277458) »
- Sachini N et al. Promyelocytic leukemia protein (PML) controls breast cancer cell proliferation by modulating Forkhead transcription factors. Mol Oncol 13:1369-1387 (2019). Read more (PubMed: 30927552) »
