Overview

  • Product name

    Anti-TATA binding protein TBP antibody - Loading Control
    See all TATA binding protein TBP primary antibodies
  • Description

    Goat polyclonal to TATA binding protein TBP - Loading Control
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog
  • Immunogen

    Synthetic peptide:

    DQNNSLPPYAQ-C

    (with a Cysteine residue linker), corresponding to N terminal amino acids 2-12 of Human TATA binding protein TBP (NP_003185.1).

  • Positive control

    • HeLa nuclear lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab134575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).

Target

  • Function

    General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.
  • Tissue specificity

    Widely expressed, with levels highest in the testis and ovary.
  • Involvement in disease

    Defects in TBP are the cause of spinocerebellar ataxia type 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.
  • Sequence similarities

    Belongs to the TBP family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • GTF2D antibody
    • GTF2D1 antibody
    • HDL4 antibody
    • MGC117320 antibody
    • MGC126054 antibody
    • MGC126055 antibody
    • SCA17 antibody
    • TATA binding factor antibody
    • TATA box factor antibody
    • TATA sequence binding protein antibody
    • TATA sequence-binding protein antibody
    • TATA-binding factor antibody
    • TATA-box binding protein N-terminal domain antibody
    • TATA-box factor antibody
    • TATA-box-binding protein antibody
    • TBP antibody
    • TBP_HUMAN antibody
    • TFIID antibody
    • Transcription initiation factor TFIID TBP subunit antibody
    see all

Images

  • All lanes : Anti-TATA binding protein TBP antibody - Loading Control (ab134575) at 0.3 µg/ml

    Lane 1 : HeLa nuclear lysate
    Lane 2 : HeLa cytosolic lysate

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 38 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

References

ab134575 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us and your interest in our products.

The samples used to perform the Western blot presented on the datasheet were not isolated using RIPA buffer. This was entered in error. I thank you for pointing this out to us. This will be amended.

The nuclear and cytoplasmic samples were isolated using a commercial kit. The extraction buffers used wereincluded in this kit, so there was no RIPA involved. If you would like to perform this isolation yourself we have the following protocol which may be of use:

https://www.abcam.com/index.html?pageconfig=resource&rid=11408

For your convenience, you could consider some of our https://www.abcam.com/index.html?c=4573such as https://www.abcam.com/Cell-Fractionation-Kit-HT-ab109718.html#Cell-Fractionation-Kits-ab109718-4.jpg.

I hope this information has been of help. If you have any further questions, please do not hesitate to ask.

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