Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TATA binding protein TBP antibody [mAbcam 51841] - Nuclear Loading Control and ChIP Grade

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver P14)
Specification
Liver P14
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
Yes - Triton 0,1%
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C

Other product details

Dilution
1/400
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS Triton 0.1% NGS 10%

Secondary antibody

Name
Non-Abcam antibody was used: CY3 GOAT ANTI MOUSE IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/200

Additional data

Additional Notes
Slides were rehydrated and then boiled for antigen retrieval in Citrate pH 6 buffer for 15min in standard microwave oven at 650W. After 30min blocking in PBS-Triton 0,1% / NGS 10% slides were incubated O/N with primary mAbcam 51841 (TBP) antibody at 1/400 dilution and ab15102 (Claudin3) antibody at 1/300 dilution at 4°C. The secondary antibody was CY3 GOAT ANTI MOUSE IgG (for TBP) and Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (for Claudin3) both used at a 1/200 dilution for 1h. Nuclei were stained with Hoechst. Slides were mounted with VECTASHIELD mounting solution.

Daniel Alpern

Verified customer

Submitted May 14 2013

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