Western blot abreview for Anti-TATA binding protein TBP antibody [mAbcam 51841] - Nuclear Loading Control and ChIP Grade

Excellent
Abreviews
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Mouse Cell lysate - whole cell (brain)
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
1 hour(s) and 30 minute(s) · Temperature: 20°C · Diluent: 5% milk in TBS-tween
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Sigma
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000

Detection

Detection method
ECL
Bands
Specific: 38 kDa
Exposure
5 minute(s) and 0 second(s)

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Submitted Aug 22 2014

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