Immunocytochemistry/ Immunofluorescence abreview for Anti-TATA binding protein TBP antibody [mAbcam 51841] - Nuclear Loading Control and ChIP Grade

Average
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Sample
Mouse Cell (MS)
Specification
MS
Permeabilization
Yes - Triton 1%
Fixative
Formaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Diluent: BSA 3% - PBS
Dilution
1/50

Secondary antibody

Name
Non-Abcam antibody was used: anti ms
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 555
Dilution
1/500

Additional data

Additional Notes
Good nuclear staining. Also cytoplasmic staining that could be removed by primary antibody dilution. TBP is detected by Alexa555 conjugated antibody (red) and nuclei by DAPI staining (blue)

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Submitted Feb 25 2015

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