Western blot abreview for Anti-TATA binding protein TBP antibody [mAbcam 51841] - Nuclear Loading Control and ChIP Grade

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - nuclear (B cells)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
30 µg
Specification
B cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Dilution
1/4000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: Anti Mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
2 second(s)
Bands
Specific: 40 kDa

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Submitted Jan 18 2017

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