Western blot abreview for Anti-TATA binding protein TBP antibody [mAbcam 51841] - Nuclear Loading Control and ChIP Grade

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (K562)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
20 µg
Specification
K562
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-T Solution
Dilution
1/1500

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Rabbit anti-Mouse IgG H&L (HRP)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 38 kDa

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Submitted May 23 2017

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