Western blot abreview for Anti-TATA binding protein TBP antibody [mAbcam 51841] - Nuclear Loading Control and ChIP Grade

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - nuclear (Renal (ccRCC))
Gel Running Conditions
Non-reduced Denaturing (10)
Loading amount
50 µg
Specification
Renal (ccRCC)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in PBS Tween (0.1%)
Dilution
1/500

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: anti-mouse HRP
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 37 kDa

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Submitted Aug 20 2018

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