Western blot abreview for Anti-TATA binding protein TBP antibody [mAbcam 51841] - Nuclear Loading Control and ChIP Grade

Excellent
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (liver total protein extract)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
30 µg
Specification
liver total protein extract
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 5% milk powder in PBS-T
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name

Detection

Detection method
ECL
Exposure
1 second(s)
Bands
Specific: 38 kDa
Positive control
My samples should all be positive for TBP. TBP was used as a loading control.

Additional data

Additional Notes
The figure shows different samples of mouse liver total protein extract and a molecular weight marker. A single band around 38kDa is observed in all samples. The TBP band detected by chemiluminescence is shown in red. The fluorescent marker is shown in green and blue.

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Submitted Oct 24 2018

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