Western blot abreview for Anti-TATA binding protein TBP antibody [mAbcam 51841] - Nuclear Loading Control and ChIP Grade

Excellent
Abreviews
Application
Western blot
Sample
Chinese hamster Cell lysate - whole cell (Chinese Hamster Ovarian (CHO) cells)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
20 µg
Specification
Chinese Hamster Ovarian (CHO) cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% non-fat milk in TBS-T
Dilution
1/1000

Secondary antibody

Dilution
1/2500
Name
Non-Abcam antibody was used: Dako Polyclonal Rabbit Anti-mouse Immunoglobulins/
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
SuperSignal™ West Pico PLUS Chemilumines
Exposure
20 second(s)
Bands
Specific: 38 kDa
Positive control
Huh7 liver cells ( observed band at 42 kDa)

Additional data

Additional Notes
1: 20 ug of whole cell lysate from Huh7 cells 2: 20 ug of whole cell lysate from CHO cells

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Submitted Dec 04 2018

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