Application
Western blot
Sample
Human Cell lysate - whole cell (Cancer cell lines)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Specification
Cancer cell lines
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA
Dilution
1/1000
Detection
Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 45 kDa
Additional data
Additional Notes
SW480 Whole Cell Lysate (lane #1) and SW620 Whole Cell Lysate (lane #2). TATA box band observed at 45KDa.
Abcam user community
Verified customer
Submitted Apr 15 2021