Recombinant Anti-TMF antibody [EPR10671(B)] (ab151702)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10671(B)] to TMF
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TMF antibody [EPR10671(B)]
See all TMF primary antibodies -
Description
Rabbit monoclonal [EPR10671(B)] to TMF -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human TMF (C terminal).
Database link: P82094 -
Positive control
- HepG2, HeLa, HT1376 cell lysate; MCF7 cells; HT1376 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10671(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151702 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 123 kDa.
|
ICC/IF | (2) |
1/100 - 1/250.
|
Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 123 kDa. |
ICC/IF
1/100 - 1/250. |
Target
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Function
Potential coactivator of the androgen receptor. Mediates STAT3 degradation. May play critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. This protein binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP). -
Domain
The Elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV]. -
Cellular localization
Cytoplasm. Nucleus. Golgi apparatus membrane. Concentrated at the budding structures localized at the tips of cisternae. - Information by UniProt
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Database links
- Entrez Gene: 7110 Human
- Omim: 601126 Human
- SwissProt: P82094 Human
- Unigene: 267632 Human
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Alternative names
- Androgen receptor coactivator 160 kDa protein antibody
- Androgen receptor-associated protein of 160 kDa antibody
- ARA160 antibody
see all
Images
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All lanes : Anti-TMF antibody [EPR10671(B)] (ab151702) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HT1376 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 123 kDa -
Intracellular flow cytometric analysis of permeabilized HT1376 cells labeling TMF with ab151702 at 1/10 dilution (red). Rabbit IgG negative control (green).
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Immunofluorescent analysis of MCF7 cells labeling TMF with ab151702 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab151702 has been referenced in 1 publication.
- Yue X et al. Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner. Commun Biol 4:1370 (2021). PubMed: 34876695