Overview

  • Product name
    Anti-Tau 13 antibody [B11E8]
  • Description
    Mouse monoclonal [B11E8] to Tau 13
  • Host species
    Mouse
  • Specificity
    The antibody recognizes 45-60 kDa human Tau-13. Reactivity to other species has not been tested.
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Tau 13 (N terminal).
    Database link: P10636

  • General notes


    The antibody recognizes 45-60 kDa isoform of human Tau-13.

Properties

Applications

Our Abpromise guarantee covers the use of ab19030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 79 kDa.
IP 1/500.
IHC-P 1/100.

Target

  • Relevance
    Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of tau is to promote tubulin polymerisation and stabilise microtubules, but it also serves to link certain signalling pathways to the cytoskeleton. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF)and neurofibrillary lesions in Alzheimer's disease (AD) brain. Hyperphosphorylation impairs the microtubule binding function of tau, resulting in the destabilisation of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Hyperphosphorylated tau is also found in a range of other central nervous system disorders. Numerous serine/threonine kinases, including GSK 3 beta, PKA, Cdk 5, and casein kinase II can phosphorylate tau.
  • Cellular localization
    Cytoplasmic and Plasma membrane
  • Database links
  • Alternative names
    • FTDP17 antibody
    • Microtubule associated protein tau antibody
    • Paired helical filament tau antibody
    • PHF tau antibody
    • PPND antibody
    • TAU antibody
    see all

Images

  • All lanes : Anti-Tau 13 antibody [B11E8] (ab19030) at 1/5000 dilution

    Lanes 1-2 : iPSC-derived neuronal culture (Human) at 2.5 µg
    Lane 3 : iPSC-derived neuronal culture (Human) at 1 µg
    Lane 4 : Whole brain homogenate

    Secondary
    All lanes : HRP conjugated Goat Anti-Mouse at 1/2500 dilution

    Predicted band size: 79 kDa

    See Abreview

References

This product has been referenced in:
  • Kim DK  et al. Molecular and functional signatures in a novel Alzheimer's disease mouse model assessed by quantitative proteomics. Mol Neurodegener 13:2 (2018). WB . Read more (PubMed: 29338754) »
  • Lathuilière A  et al. Motifs in the tau protein that control binding to microtubules and aggregation determine pathological effects. Sci Rep 7:13556 (2017). ELISA ; Human . Read more (PubMed: 29051562) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (iPSC-derived neuronal culture)
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Loading amount
2.5 µg
Specification
iPSC-derived neuronal culture
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Martin Engel

Verified customer

Submitted Nov 02 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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