Anti-Tau Alzheimer’s Disease antibody [GT-38] - BSA and Azide free (ab254274)

Overview

  • Product name

    Anti-Tau Alzheimer’s Disease antibody [GT-38] - BSA and Azide free
    See all Tau Alzheimer’s Disease primary antibodies
  • Description

    Mouse monoclonal [GT-38] to Tau Alzheimer’s Disease - BSA and Azide free
  • Host species

    Mouse
  • Specificity

    Mouse monoclonal [GT-38] selectively detects Alzheimer’s disease (AD) conformation of tau but does not detect tau pathology characteristic of frontotemporal lobar degeneration-tau (FTLD-tau) including corticobasal degeneration, progressive supranuclear palsy, and Pick’s disease. GT-38 differentiates AD tau pathology in the context of co-morbid FTLD-tau by immunohistochemical staining. GT-38 requires the presence of both 3R and 4R tau isoforms. GT-38 has been used in the literature as an ELISA capture antibody to detect AD brain derived PHFs with total tau reporter antibodies (PubMed ID: 29415231).

  • Tested applications

    Suitable for: ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Other Immunogen Type corresponding to Human Tau Alzheimer’s Disease. Tau paired helical filaments extracted from Alzheimer disease human brain tissue

  • Positive control

    • IHC-P: FFPE Human Alzheimer brain tissue sections.
  • General notes

    ab254274 is a PBS only version of ab246808.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    GT-38
  • Isotype

    IgG1
  • Light chain type

    kappa

Applications

Our Abpromise guarantee covers the use of ab254274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

GT-38 works as a capture antibody but we have not had success using it as a detection antibody and have not explored extensive antibody sandwich partner compatibility.

IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Alternative names

    • Tau neurofibrillary tangles antibody

Images

  • This image was developed using the same antibody clone but in a different formulation, containing PBS and sodium azide, ab246808.

    IHC image of Tau Alzheimer’s Disease staining in a section of formalin-fixed paraffin-embedded human Alzheimer brain performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab246808, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • This image was developed using the same antibody clone but in a different formulation, containing PBS and sodium azide, ab246808.

    IHC image of Tau Alzheimer’s Disease staining in a section of formalin-fixed paraffin-embedded human normal brain performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab246808, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • GT-38 ELISA selectively detects AD-tau compared to tau monomer. GT-38 immobilized antibody selectively captures pathological tau derived from human AD brain tissue compared to recombinant tau monomer. GT-38 captured AD-tau antigens are detected with total tau rabbit polyclonal antibodies followed by HRP-conjugated anti-rabbit secondary and colorimetric readout. Total tau ELISA serves as a loading control demonstrating equivalent loading of total tau protein from both AD-tau and tau monomer.

    Purified GT-38 mouse IgG1 antibody was coated on a 384 well plate at 450 ng/well (30 uL of 15 ng/uL) in 0.1 M NaHCO3 pH 9.6 at 4°C overnight. Plates were washed with PBST (phosphate buffered saline pH 7.2 with 0.05% Tween20) five times. GT-38 capture antibody was blocked with a commercial blocking solution 4°C overnight. Antigens consisted of either human brain derived insoluble tau enriched extract (AD-tau) or purified recombinant tau monomer. AD-tau was sonicated with a probe sonicator and antigens diluted in 0.2% BSA in PBS and applied to immobilized GT-38 and allowed to incubate 4°C overnight. Plates were washed with PBST five times. Rabbit polyclonal total tau detection antibody was diluted to 3.7 μg/mL in Buffer C (20 mM sodium phosphate pH 7.0, 2 mM EDTA, 400 mM sodium chloride, 1% BSA, 0.005% thimerosal), 30 μL was added to each well and incubated for 1 hour at room temperature. Plates were washed with PBST five times. Anti-rabbit HRP conjugated was diluted 1: 3,000 in Buffer C, 30 μL was added to each well and incubated for 1 hour at RT. Plates were washed with PBST five times. Colorimetric HRP substrate (30 μL/well) was added to the plate and developed at RT for 10 minutes. The reaction was quenched with addition of 30 μL/well 10% H2PO4 and the absorbance was measured at wavelength 450 nm.

    This image was developed using the same antibody clone but in a different formulation, containing PBS and sodium azide, ab246808.

     

References

This product has been referenced in:

  • Gibbons GS  et al. Detection of Alzheimer's disease (AD) specific tau pathology with conformation-selective anti-tau monoclonal antibody in co-morbid frontotemporal lobar degeneration-tau (FTLD-tau). Acta Neuropathol Commun 7:34 (2019). IHC-P ; Human . Read more (PubMed: 30832741) »
  • Gibbons GS  et al. Detection of Alzheimer Disease (AD)-Specific Tau Pathology in AD and NonAD Tauopathies by Immunohistochemistry With Novel Conformation-Selective Tau Antibodies. J Neuropathol Exp Neurol 77:216-228 (2018). IHC-P, Dot blot, Sandwich ELISA ; Human . Read more (PubMed: 29415231) »
See all 2 Publications for this product

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