Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (ab246808)

Overview

  • Product name

    Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific
    See all Tau Alzheimer’s Disease primary antibodies
  • Description

    Mouse monoclonal [GT-38] to Tau Alzheimer’s Disease - Conformation-Specific
  • Host species

    Mouse
  • Specificity

    Mouse monoclonal [GT-38] selectively detects Alzheimer’s disease (AD) conformation of tau but does not detect tau pathology characteristic of frontotemporal lobar degeneration-tau (FTLD-tau) including corticobasal degeneration, progressive supranuclear palsy, and Pick’s disease. GT-38 differentiates AD tau pathology in the context of co-morbid FTLD-tau by immunohistochemical staining. GT-38 requires the presence of both 3R and 4R tau isoforms. GT-38 has been used in the literature as an ELISA capture antibody to detect AD brain derived PHFs with total tau reporter antibodies (PubMed ID: 29415231).

  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Other Immunogen Type corresponding to Human Tau Alzheimer’s Disease. Tau paired helical filaments extracted from Alzheimer disease human brain tissue

  • Positive control

    • IHC-P: FFPE Human Alzheimer brain tissue sections.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    GT-38
  • Isotype

    IgG1
  • Light chain type

    kappa

Applications

Our Abpromise guarantee covers the use of ab246808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Alternative names

    • Tau neurofibrillary tangles antibody

Images

  • IHC image of Tau Alzheimer’s Disease staining in a section of formalin-fixed paraffin-embedded human Alzheimer brain performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab246808, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • IHC image of Tau Alzheimer’s Disease staining in a section of formalin-fixed paraffin-embedded human normal brain performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab246808, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • Gibbons GS  et al. Detection of Alzheimer's disease (AD) specific tau pathology with conformation-selective anti-tau monoclonal antibody in co-morbid frontotemporal lobar degeneration-tau (FTLD-tau). Acta Neuropathol Commun 7:34 (2019). IHC-P, IF ; Human . Read more (PubMed: 30832741) »
  • Gibbons GS  et al. Detection of Alzheimer Disease (AD)-Specific Tau Pathology in AD and NonAD Tauopathies by Immunohistochemistry With Novel Conformation-Selective Tau Antibodies. J Neuropathol Exp Neurol 77:216-228 (2018). IHC-P, Dot blot, Sandwich ELISA ; Human . Read more (PubMed: 29415231) »
See all 2 Publications for this product

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