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  1. Link

    tau-alzheimers-disease-antibody-gt-38-conformation-specific-ab246808.pdf

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Neuroscience Neurology process Neurodegenerative disease Alzheimer's disease Tangles & Tau
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Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (ab246808)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (ab246808)
  • Immunohistochemistry paraffin embedded sections - Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (ab246808)
  • ELISA - Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (ab246808)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (ab246808)
  • Immunohistochemistry (Frozen sections) - Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (ab246808)

Key features and details

  • Mouse monoclonal [GT-38] to Tau Alzheimer’s Disease - Conformation-Specific
  • Suitable for: IHC-P, ELISA, IHC-P, IHC-Fr
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific
    See all Tau Alzheimer’s Disease primary antibodies
  • Description

    Mouse monoclonal [GT-38] to Tau Alzheimer’s Disease - Conformation-Specific
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, ELISA, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Other Immunogen Type corresponding to Human Tau Alzheimer’s Disease. Tau paired helical filaments extracted from Alzheimer disease human brain tissue.

  • Positive control

    • IHC-P: FFPE and Frozen Human Alzheimer brain tissue sections.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    GT-38
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Tangles & Tau
    • Neuroscience
    • Diseases

Associated products

  • Alternative Versions

    • Anti-Tau Alzheimer’s Disease antibody [GT-38] - BSA and Azide free (ab254274)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab246808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.

GT-38 works as a capture antibody but we have not had success using it as a detection antibody and have not explored extensive antibody sandwich partner compatibility.

IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.
Notes
IHC-P
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.

GT-38 works as a capture antibody but we have not had success using it as a detection antibody and have not explored extensive antibody sandwich partner compatibility.

IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.

Target

  • Relevance

    Function: Tau promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. Tissue specificity: Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system. Involvement in disease: Polymerization of tau into insoluble aggregates within the CNS is a pathological hallmark of a clinically heterogeneous set of neurodegenerative diseases termed tauopathies, including Alzheimer disease (AD), corticobasal degenerations (CBD), progressive supranuclear palsy (PSP), and Pick’s disease (PiD). Tau isoforms are differentially incorporated into insoluble fibrillary structures in distinct tauopathies: for instance, in PSP and CBD 4R (microtubule binding repeat) isoforms are mostly incorporated in inclusion bodies, while in PiD 3R tau isoforms are predominant. Neuronal tau inclusions observed in AD consist primarily of both 3R and 4R isoforms, aggregated into hyperphosphorylated straight filaments (SF) and paired helical filaments (PHF). The resulting neurofibrillary tangles cause the loss of tau function of promoting assembly and stabilizing microtubules, and also the gain of a toxic function whereby the pathological tau sequesters normal tau, MAP1A/MAP1B and MAP2, and causes inhibition and disruption of microtubules. Sequence similarities: Four-repeat (type II) tau is expressed in an adult-specific manner and is not found in fetal brain, whereas three-repeat (type I) tau is found in both adult and fetal brain. Domain: The tau/MAP repeat binds to tubulin. Type I isoforms contain 3 repeats while type II isoforms contain 4 repeats.
  • Database links

    • Clone ID: 4137 Human
    • Omim: 157140 Human
    • SwissProt: P10636 Human
    • Alternative names

      • Alzheimer disease specific antibody
      • G protein beta1/gamma2 subunit interacting factor 1 antibody
      • MAPT antibody
      • MAPTL antibody
      • Microtubule associated protein tau antibody
      • MSTD antibody
      • Mtapt antibody
      • MTBT1 antibody
      • MTBT2 antibody
      • Neurofibrillary tangle protein antibody
      • Paired helical filament tau antibody
      • PHF tau antibody
      • TAU antibody
      • Tau neurofibrillary tangles antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (ab246808)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (ab246808)

      IHC image of Tau Alzheimer’s Disease staining in a section of formalin-fixed paraffin-embedded human Alzheimer brain performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab246808, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Immunohistochemistry paraffin embedded sections - Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (ab246808)
      Immunohistochemistry paraffin embedded sections - Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (ab246808)This data and image were courteously provided by Dr. Garrett Gibbons, University of Pennsylvania

      GT-38 detects Alzheimer's disease (AD) tau pathology. Immunofluorescence staining with GT-38 (green) at 2 µg/mL followed by anti mouse-488 conjugated secondary detection antibody in 6 µm thick sections of paraffin embedded human AD hippocampus fixed in ethanol. Blue stain is DAPI positive nuclei.

    • ELISA - Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (ab246808)
      ELISA - Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (ab246808)This data and image were courteously provided by Dr. Garrett Gibbons, University of Pennsylvania.

      GT-38 ELISA selectively detects AD-tau compared to tau monomer. GT-38 immobilized antibody selectively captures pathological tau derived from human AD brain tissue compared to recombinant tau monomer. GT-38 captured AD-tau antigens are detected with total tau rabbit polyclonal antibodies followed by HRP-conjugated anti-rabbit secondary and colorimetric readout. Total tau ELISA serves as a loading control demonstrating equivalent loading of total tau protein from both AD-tau and tau monomer.

      Purified GT-38 mouse IgG1 antibody was coated on a 384 well plate at 450 ng/well (30 uL of 15 ng/uL) in 0.1 M NaHCO3 pH 9.6 at 4°C overnight. Plates were washed with PBST (phosphate buffered saline pH 7.2 with 0.05% Tween20) five times. GT-38 capture antibody was blocked with a commercial blocking solution 4°C overnight. Antigens consisted of either human brain derived insoluble tau enriched extract (AD-tau) or purified recombinant tau monomer. AD-tau was sonicated with a probe sonicator and antigens diluted in 0.2% BSA in PBS and applied to immobilized GT-38 and allowed to incubate 4°C overnight. Plates were washed with PBST five times. Rabbit polyclonal total tau detection antibody was diluted to 3.7 μg/mL in Buffer C (20 mM sodium phosphate pH 7.0, 2 mM EDTA, 400 mM sodium chloride, 1% BSA, 0.005% thimerosal), 30 μL was added to each well and incubated for 1 hour at room temperature. Plates were washed with PBST five times. Anti-rabbit HRP conjugated was diluted 1: 3,000 in Buffer C, 30 μL was added to each well and incubated for 1 hour at RT. Plates were washed with PBST five times. Colorimetric HRP substrate (30 μL/well) was added to the plate and developed at RT for 10 minutes. The reaction was quenched with addition of 30 μL/well 10% H2PO4 and the absorbance was measured at wavelength 450 nm.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (ab246808)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (ab246808)

      IHC image of Tau Alzheimer’s Disease staining in a section of formalin-fixed paraffin-embedded human normal brain performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab246808, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Immunohistochemistry (Frozen sections) - Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (ab246808)
      Immunohistochemistry (Frozen sections) - Anti-Tau Alzheimer’s Disease antibody [GT-38] - Conformation-Specific (ab246808)

      IHC image of Tau Alzheimer’s Disease staining in a section of frozen human Alzheimer brain performed on a Leica BONDTM system using the standard protocol F. The section was incubated with ab246808, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (4)

    Publishing research using ab246808? Please let us know so that we can cite the reference in this datasheet.

    ab246808 has been referenced in 4 publications.

    • Arena JD  et al. Tau immunophenotypes in chronic traumatic encephalopathy recapitulate those of ageing and Alzheimer's disease. Brain 143:1572-1587 (2020). IHC ; Human . PubMed: 32390044
    • He Z  et al. Transmission of tauopathy strains is independent of their isoform composition. Nat Commun 11:7 (2020). IHC ; Human . PubMed: 31911587
    • Gibbons GS  et al. Detection of Alzheimer's disease (AD) specific tau pathology with conformation-selective anti-tau monoclonal antibody in co-morbid frontotemporal lobar degeneration-tau (FTLD-tau). Acta Neuropathol Commun 7:34 (2019). IHC-P, IF ; Human . PubMed: 30832741
    • Gibbons GS  et al. Detection of Alzheimer Disease (AD)-Specific Tau Pathology in AD and NonAD Tauopathies by Immunohistochemistry With Novel Conformation-Selective Tau Antibodies. J Neuropathol Exp Neurol 77:216-228 (2018). IHC-P, Dot blot, Sandwich ELISA ; Human . PubMed: 29415231

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