Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tau antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Specification
Colon
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Other product details

Dilution
1/4000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide

Secondary antibody

Name
Non-Abcam antibody was used: Anti-Sheep IgG
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
Submitted image is of the junction between the inner circular and the longitudinal smooth muscle of the Muscularis externa.
Red arrowheads show two Ganglia of Auerbach's plexus.
Green arrowheads indicate positivity of individual nerve fibres throughtout the muscle.

Mr. Carl Hobbs

Verified customer

Submitted Feb 24 2011

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