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Our Abpromise guarantee covers the use of ab64193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 79 kDa).|
|Dot blot||Use a concentration of 0.5 - 2 µg/ml. By dot blot, this antibody only recognizes the immunizing peptide.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration.|
Immunohistochemical analysis of Apteronotus leptorhynchus brain tissue, staining Tau with ab64193.
Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 3% sheep serum (ab7489), 1% BSA and 1% teleostean gelatine in TBS for 1 hour at 24°C. Samples were incubated with primary antibody (1/20 in blocking solution) for 18 hours at 4°C. An AlexaFluor®546-conjugated goat anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody.
Immunocytochemistry of Zebrafish Cultured Cells (primary neuron), labelling Tau with ab64193.
Immunocytochemistry/ Immunofluorescence analysis of mouse primary cortical neuronal cells abeling Tau with ab64193 at a dilution of 1/100. The cells were fixed with Ethanol and permeabilized with 0.2% Triton X-100. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody.
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