Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Tau antibody

Poor
Abreviews
This product is known to not work in this application or species.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid HIER
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Other product details

Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide

Secondary antibody

Name
Non-Abcam antibody was used: anti rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200

Additional data

Additional Notes
Practically every nucleus appears to be positive. No cytoplasmic/fibre positivity is observed in any brain area in this parasagittal plane. Submitted image is of the striatum and lateral ventricle, showing the svz containing nests of progenitor neurones ( green arowheads)
Abcam response
Many thanks for your feedback on ab64193, we regret that it has not performed well for you in IHC-P on mouse brain tissue; your data would suggest that this antibody is not suitable for use in IHC-P.

Mr. Carl Hobbs

Verified customer

Submitted Nov 02 2010

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