Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Tau antibody

Good
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Apteronotus leptorhynchus Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C

Other product details

Dilution
1/20
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking solution

Secondary antibody

Name
Non-Abcam antibody was used: Alexa 546-conjugated goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 546
Dilution
1/200

Additional data

Additional Notes
The antibody specifically labels cell bodies and axons of neurons throughout the brain.

Abcam user community

Verified customer

Submitted Nov 26 2012

Sign up