Western blot abreview for Anti-Tau antibody

Good
Abreviews
Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Dilution
1/1000
Incubation time
4 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: 2.5% milk in PBS-Tween20

Secondary antibody

Name
Non-Abcam antibody was used: Alexa 488-conjugated goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000

Detection

Detection method
fluorescence scanning
Exposure
7 minute(s) and 0 second(s)
Bands
Specific: 50 kDa Non-specific: 45 kDa

Additional data

Additional Notes
The secondary antibody (Alexa488-conjugated) was detected using a fluorescence scanner equipped with a green laser (532 nm), at a PMT of 750V.

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Submitted Nov 26 2012

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