Western blot abreview for Anti-Tau antibody

Excellent
Abreviews
Application
Western blot
Loading amount
120 µg
Gel Running Conditions
Reduced Denaturing (15)
Sample
Mouse Tissue lysate - whole (Brain)
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5%milk+ PBS+0.1% Tween 20
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Bands
Specific: 52 kDa
Exposure
5 minute(s) and 0 second(s)

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Submitted Apr 16 2014

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