• Product name
  • Description
    Chicken polyclonal to Tau
  • Host species
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Two synthetic peptides corresponding to two regions of the Tau gene product corresponding to sequences shared between the mouse (P01637, NCBI) and human (NP_05819, NCBI) proteins, and not containing any of the serine and threonine residues known to undergo phosphorylation.

  • Positive control
    • Hippocampus neurons of a patient with Alzheimer's disease. This antibody gave a positive result in IHC in the following FFPE tissue: Human Hippocampus (Alzheimer's).
  • General notes

    Two different affinity purified anti peptide antibodies were combined to make this product. The concentrations of both of these antibodies was 100 µg/ml, making the total antibody concentration 200 µg/ml.



Our Abpromise guarantee covers the use of ab75714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/20000 - 1/50000. Predicted molecular weight: 79 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/1000 - 1/2000.


  • Function
    Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
  • Tissue specificity
    Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.
  • Involvement in disease
    Note=In Alzheimer disease, the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments (PHF) and straight filaments, mainly composed of hyperphosphorylated forms of TAU (PHF-TAU or AD P-TAU).
    Defects in MAPT are a cause of frontotemporal dementia (FTD) [MIM:600274]; also called frontotemporal dementia (FTD), pallido-ponto-nigral degeneration (PPND) or historically termed Pick complex. This form of frontotemporal dementia is characterized by presenile dementia with behavioral changes, deterioration of cognitive capacities and loss of memory. In some cases, parkinsonian symptoms are prominent. Neuropathological changes include frontotemporal atrophy often associated with atrophy of the basal ganglia, substantia nigra, amygdala. In most cases, protein tau deposits are found in glial cells and/or neurons.
    Defects in MAPT are a cause of Pick disease of the brain (PIDB) [MIM:172700]. It is a rare form of dementia pathologically defined by severe atrophy, neuronal loss and gliosis. It is characterized by the occurrence of tau-positive inclusions, swollen neurons (Pick cells) and argentophilic neuronal inclusions known as Pick bodies that disproportionally affect the frontal and temporal cortical regions. Clinical features include aphasia, apraxia, confusion, anomia, memory loss and personality deterioration.
    Note=Defects in MAPT are a cause of corticobasal degeneration (CBD). It is marked by extrapyramidal signs and apraxia and can be associated with memory loss. Neuropathologic features may overlap Alzheimer disease, progressive supranuclear palsy, and Parkinson disease.
    Defects in MAPT are a cause of progressive supranuclear palsy type 1 (PSNP1) [MIM:601104, 260540]; also abbreviated as PSP and also known as Steele-Richardson-Olszewski syndrome. PSNP1 is characterized by akinetic-rigid syndrome, supranuclear gaze palsy, pyramidal tract dysfunction, pseudobulbar signs and cognitive capacities deterioration. Neurofibrillary tangles and gliosis but no amyloid plaques are found in diseased brains. Most cases appear to be sporadic, with a significant association with a common haplotype including the MAPT gene and the flanking regions. Familial cases show an autosomal dominant pattern of transmission with incomplete penetrance; genetic analysis of a few cases showed the occurrence of tau mutations, including a deletion of Asn-613.
  • Sequence similarities
    Contains 4 Tau/MAP repeats.
  • Developmental stage
    Four-repeat (type II) tau is expressed in an adult-specific manner and is not found in fetal brain, whereas three-repeat (type I) tau is found in both adult and fetal brain.
  • Domain
    The tau/MAP repeat binds to tubulin. Type I isoforms contain 3 repeats while type II isoforms contain 4 repeats.
  • Post-translational
    Phosphorylation at serine and threonine residues in S-P or T-P motifs by proline-directed protein kinases (PDPK: CDK1, CDK5, GSK-3, MAPK) (only 2-3 sites per protein in interphase, seven-fold increase in mitosis, and in PHF-tau), and at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK) in Alzheimer diseased brains. Phosphorylation decreases with age. Phosphorylation within tau's repeat domain or in flanking regions seems to reduce tau's interaction with, respectively, microtubules or plasma membrane components. Phosphorylation on Ser-610, Ser-622, Ser-641 and Ser-673 in several isoforms during mitosis.
    Polyubiquitinated. Requires functional TRAF6 and may provoke SQSTM1-dependent degradation by the proteasome (By similarity). PHF-tau can be modified by three different forms of polyubiquitination. 'Lys-48'-linked polyubiquitination is the major form, 'Lys-6'-linked and 'Lys-11'-linked polyubiquitination also occur.
    Glycation of PHF-tau, but not normal brain tau. Glycation is a non-enzymatic post-translational modification that involves a covalent linkage between a sugar and an amino group of a protein molecule forming ketoamine. Subsequent oxidation, fragmentation and/or cross-linking of ketoamine leads to the production of advanced glycation endproducts (AGES). Glycation may play a role in stabilizing PHF aggregation leading to tangle formation in AD.
  • Cellular localization
    Cytoplasm > cytosol. Cell membrane. Cytoplasm > cytoskeleton. Cell projection > axon. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.
  • Information by UniProt
  • Database links
  • Form
    There are 9 isoforms produced by alternative splicing.
  • Alternative names
    • AI413597 antibody
    • AW045860 antibody
    • DDPAC antibody
    • FLJ31424 antibody
    • FTDP 17 antibody
    • G protein beta1/gamma2 subunit interacting factor 1 antibody
    • MAPT antibody
    • MAPTL antibody
    • MGC134287 antibody
    • MGC138549 antibody
    • MGC156663 antibody
    • Microtubule associated protein tau antibody
    • Microtubule associated protein tau isoform 4 antibody
    • Microtubule-associated protein tau antibody
    • MSTD antibody
    • Mtapt antibody
    • MTBT1 antibody
    • MTBT2 antibody
    • Neurofibrillary tangle protein antibody
    • Paired helical filament tau antibody
    • Paired helical filament-tau antibody
    • PHF tau antibody
    • PHF-tau antibody
    • PPND antibody
    • PPP1R103 antibody
    • Protein phosphatase 1, regulatory subunit 103 antibody
    • pTau antibody
    • RNPTAU antibody
    • TAU antibody
    • TAU_HUMAN antibody
    • Tauopathy and respiratory failure, included antibody
    see all


  • ab75714 staining Tau in rat hippocampal neurons by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using 0.25% Triton, blocked with 3% BSA for 30 minutes at room temperature and then incubated with ab75714 at a 1/1000 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-chicken polyclonal used at a 1/1000 dilution.

    Staining in rat neurons is great. Hippocampal cultured neurons (DIV7) were labelled against tau (green), tubulin (red) and DAPI (blue).

    See Abreview

  • Immunohistochemistry (10% Formalin-fixed paraffin-embedded sections) analysis of Human Alzheimer's disease brain (CA1 region of hippocampus) tissue labelling Tau (brown) with ab75714.

  • Immunofluorescence analysis of primary entorhinal cortex neurons prepared from five-day-old rats, staining Tau with ab75714 at 1/1500 dilution.

    Cells were fixed with paraformaldehyde, blocked in PBS + 5% fetal calf serum (ab7479), then permeabilized in PBS containing 0.2% Triton X-100. Samples were incubated with primary antibody for 2 hours at room temperature. A Cy5®-conjugated anti-chicken IgG was used as the secondary antibody.

  • IHC image of Tau staining in Human Hippocampus (Alzheimer's) (ab4583) formalin-fixed paraffin-embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75714, 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.


    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:
  • Illouz T  et al. Restoring microglial and astroglial homeostasis using DNA immunization in a Down Syndrome mouse model. Brain Behav Immun 75:163-180 (2019). Read more (PubMed: 30389461) »
  • Mencarelli C  et al. RanBP1 Couples Nuclear Export and Golgi Regulation through LKB1 to Promote Cortical Neuron Polarity. Cell Rep 24:2529-2539.e4 (2018). Read more (PubMed: 30184488) »
See all 14 Publications for this product

Customer reviews and Q&As

1-10 of 11 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence
Human Cultured Cells (neurons derived from iPSCs)
Yes - 0.25% Triton X-100 in PBS
neurons derived from iPSCs

Abcam user community

Verified customer

Submitted Jun 20 2017

Immunohistochemistry (Frozen sections)
Mouse Tissue sections (Brain)
Yes - 0.25% triton in PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 22°C

Dr. Eitan Okun

Verified customer

Submitted May 16 2016

Immunocytochemistry/ Immunofluorescence
Human Cell (neurons derived from iPSCs)
Yes - 0.25% Triton X-100 in PBS
neurons derived from iPSCs
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Apr 15 2016

Mouse Cultured Cells (Stem Cell Derived Mouse Neurons)
Yes - .1% Saponin and tried .25% TritonX
Stem Cell Derived Mouse Neurons
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 11 2015


This is confusing. ab113316 is a kit not antibody, customer would not be requiring a DAB kit for this.

For ab101854, ab80436 will be a good product.


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Thank you for contacting us.

The kits ab80436 and ab64264, both will be OK to use with ab10148 only because it is rabbit antibody, you can select any one of them. Please note that these kits cannot be used with ab75714 because it is a chicken polyclonal antibody - you would require a anti chicken HRP conjugated antibody.

There are two possibilities one you change ab75714 with mouse or rabbit as host, second buy the HRP conjugated anti rabbit and anti chicken secondary antibodies including DAB reagents.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More


Thank you for contacting us.

The Tau antibody was raised against two distinct peptides corresponding to identical sequences shared in the mouse and human gene product.

These peptides are located in the C-terminal third of the protein.This region is away from the phosphorylated serine and threonine residues found in the N-terminal half.
Both peptides regions are found within the tubulin binding domains.

Please let me know if I can be of further assistance.

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Thank you very much for your interest in ab75714.
To our knowledge, ab75714 has not been tested in Zebrafish. Therefore, I can offer a discount off a future purchase if you buy ab75714 now, test it in Zebrafish and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free PRIMARY ANTIBODY.
If you are interested in this offer, please follow these steps:
1. Reply to this e-mail to let me know that you would like to proceed and test ab75714 in Zebrafish. I will then send a discount code. This code must be issued before purchasing ab75714 so please wait for my reply before ordering.
2. Purchase ab75714 either by phone, fax, or online (www.abcam.com).
3. Test it in Zebrafish.
4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.
We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab75714 turns out to be unsuitable for Zebrafish, you will still receive the discount on your next purchase after your Abreview has been submitted.
Please let me know if you have any questions about this offer and I would be happy to help you further.
The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Thank you for your inquiry.

For ab58201, we have 2 customer submitted Abreviews showing the antibody working in immunocytochemistry (ICC/IF), onewith rat glioma cells and the other with human glioblastoma cells. However, we don't have any publications.



As for the Abreview image for ab75714, that is a customer submitted photo, so unfortunately we do not have another version.


I am sorry that this information could not be more helpful but please contact us with any other questions.

Read More
Rat Cultured Cells (hippocampal neurons)
hippocampal neurons
Yes - triton 0,25%
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C

Maria Joana

Verified customer

Submitted Jan 04 2012

1-10 of 11 Abreviews or Q&A

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