Western blot abreview for Anti-Tau antibody [TAU-5]

Inconclusive
Abreviews
Application
Western blot
Sample
Zebrafish Tissue lysate - whole (whole fish)
Loading amount
10 µg
Specification
whole fish
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: DaKo Goat Anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Positive control
Transgenic zebrafish expressing human Tau

Additional data

Additional Notes
The antibody clearly recognizes human Tau expressed in zebrafish but It also gives many unspecific bands that could be endogenous zebrafish Tau according to the size (50-75 KDa aprox), but there are others that seem unspecific so it is difficult to know.
Abcam response
Thank you for participating in Abtrial testing. The antibody is guaranteed in WB using human samples. Zebrafish on this occasion did not work, unfortunately.

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Submitted Nov 27 2015

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