Western blot abreview for Anti-Tau antibody [TAU-5]

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Hippocampus and cerebral cortex)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
Hippocampus and cerebral cortex
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in TBS-Tween 0.1%
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti-Mouse IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
Clarity™ Western ECL Substrate
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 50-60 kDa

Additional data

Additional Notes
Lane 1 and 2: Mouse hippocampus (Whole tissue lysate), 2 different samples. Lane 3 and 4: Mouse cerebral cortex (Whole tissue lysate), 2 different samples.

Dr. Sergi Bayod

Verified customer

Submitted Sep 05 2016

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