Western blot abreview for Anti-Tau antibody [TAU-5] - BSA and Azide free

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - nuclear (hypocampus)
Gel Running Conditions
Non-reduced Denaturing (NuPage Gels 4-12% BT gels))
Loading amount
20 µg
Specification
hypocampus
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
1 hour(s) and 30 minute(s) · Temperature: 22°C · Diluent: 1x TBS + 0.1% Tween
Dilution
1/1000

Secondary antibody

Dilution
1/20000
Name
Non-Abcam antibody was used: goat anti-mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ecl
Negative control
mock transfection, but this isn't shown on this image
Exposure
30 second(s)
Bands
Specific: aprox 50-75 kDa Non-specific: 30? kDa
Positive control
WT

Additional data

Additional Notes
A very good antibody and doesn't have that many non-specific bands, compared to the similar antibody of other brands.

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Submitted Aug 31 2017

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