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Neuroscience Neurology process Neurodegenerative disease Alzheimer's disease Tangles & Tau
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Anti-Tau (phospho S199) antibody (ab4749)

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Western blot - Anti-Tau (phospho S199) antibody (ab4749)

    Key features and details

    • Rabbit polyclonal to Tau (phospho S199)
    • Suitable for: WB
    • Reacts with: African green monkey
    • Isotype: IgG

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    View more associated products

    Overview

    • Product name

      Anti-Tau (phospho S199) antibody
      See all Tau primary antibodies
    • Description

      Rabbit polyclonal to Tau (phospho S199)
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      African green monkey
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Human Tau.
      Database link: P10636
      (Peptide available as ab5241)

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.30
      Preservative: 0.05% Sodium azide
      Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated tau. The final product is generated by affinity chromatography using a tau-derived peptide that is phosphorylated at serine 199.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Alzheimer's disease
      • Tangles & Tau
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Microtubules
      • MT Associated Proteins
      • Tau
      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Other
      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Axon marker

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human Tau (mutated P301S) protein (Active) (ab246005)
    • Related Products

      • Human Tau ELISA Kit (ab210972)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab4749 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    African green monkey
    All applications
    Rat
    Application Abreviews Notes
    WB
    1/1000. Detects a band of approximately 60 kDa.
    Notes
    WB
    1/1000. Detects a band of approximately 60 kDa.

    Target

    • Function

      Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
    • Tissue specificity

      Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.
    • Involvement in disease

      Note=In Alzheimer disease, the neuronal cytoskeleton in the brain is progressively disrupted and replaced by tangles of paired helical filaments (PHF) and straight filaments, mainly composed of hyperphosphorylated forms of TAU (PHF-TAU or AD P-TAU).
      Defects in MAPT are a cause of frontotemporal dementia (FTD) [MIM:600274]; also called frontotemporal dementia (FTD), pallido-ponto-nigral degeneration (PPND) or historically termed Pick complex. This form of frontotemporal dementia is characterized by presenile dementia with behavioral changes, deterioration of cognitive capacities and loss of memory. In some cases, parkinsonian symptoms are prominent. Neuropathological changes include frontotemporal atrophy often associated with atrophy of the basal ganglia, substantia nigra, amygdala. In most cases, protein tau deposits are found in glial cells and/or neurons.
      Defects in MAPT are a cause of Pick disease of the brain (PIDB) [MIM:172700]. It is a rare form of dementia pathologically defined by severe atrophy, neuronal loss and gliosis. It is characterized by the occurrence of tau-positive inclusions, swollen neurons (Pick cells) and argentophilic neuronal inclusions known as Pick bodies that disproportionally affect the frontal and temporal cortical regions. Clinical features include aphasia, apraxia, confusion, anomia, memory loss and personality deterioration.
      Note=Defects in MAPT are a cause of corticobasal degeneration (CBD). It is marked by extrapyramidal signs and apraxia and can be associated with memory loss. Neuropathologic features may overlap Alzheimer disease, progressive supranuclear palsy, and Parkinson disease.
      Defects in MAPT are a cause of progressive supranuclear palsy type 1 (PSNP1) [MIM:601104, 260540]; also abbreviated as PSP and also known as Steele-Richardson-Olszewski syndrome. PSNP1 is characterized by akinetic-rigid syndrome, supranuclear gaze palsy, pyramidal tract dysfunction, pseudobulbar signs and cognitive capacities deterioration. Neurofibrillary tangles and gliosis but no amyloid plaques are found in diseased brains. Most cases appear to be sporadic, with a significant association with a common haplotype including the MAPT gene and the flanking regions. Familial cases show an autosomal dominant pattern of transmission with incomplete penetrance; genetic analysis of a few cases showed the occurrence of tau mutations, including a deletion of Asn-613.
    • Sequence similarities

      Contains 4 Tau/MAP repeats.
    • Developmental stage

      Four-repeat (type II) tau is expressed in an adult-specific manner and is not found in fetal brain, whereas three-repeat (type I) tau is found in both adult and fetal brain.
    • Domain

      The tau/MAP repeat binds to tubulin. Type I isoforms contain 3 repeats while type II isoforms contain 4 repeats.
    • Post-translational
      modifications

      Phosphorylation at serine and threonine residues in S-P or T-P motifs by proline-directed protein kinases (PDPK: CDK1, CDK5, GSK-3, MAPK) (only 2-3 sites per protein in interphase, seven-fold increase in mitosis, and in PHF-tau), and at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK) in Alzheimer diseased brains. Phosphorylation decreases with age. Phosphorylation within tau's repeat domain or in flanking regions seems to reduce tau's interaction with, respectively, microtubules or plasma membrane components. Phosphorylation on Ser-610, Ser-622, Ser-641 and Ser-673 in several isoforms during mitosis.
      Polyubiquitinated. Requires functional TRAF6 and may provoke SQSTM1-dependent degradation by the proteasome (By similarity). PHF-tau can be modified by three different forms of polyubiquitination. 'Lys-48'-linked polyubiquitination is the major form, 'Lys-6'-linked and 'Lys-11'-linked polyubiquitination also occur.
      Glycation of PHF-tau, but not normal brain tau. Glycation is a non-enzymatic post-translational modification that involves a covalent linkage between a sugar and an amino group of a protein molecule forming ketoamine. Subsequent oxidation, fragmentation and/or cross-linking of ketoamine leads to the production of advanced glycation endproducts (AGES). Glycation may play a role in stabilizing PHF aggregation leading to tangle formation in AD.
    • Cellular localization

      Cytoplasm > cytosol. Cell membrane. Cytoplasm > cytoskeleton. Cell projection > axon. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.
    • Target information above from: UniProt accession P10636 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 29477 Rat
      • SwissProt: P19332 Rat
      • Unigene: 2455 Rat
      • Form

        There are 9 isoforms produced by alternative splicing.
      • Alternative names

        • AI413597 antibody
        • AW045860 antibody
        • DDPAC antibody
        • FLJ31424 antibody
        • FTDP 17 antibody
        • G protein beta1/gamma2 subunit interacting factor 1 antibody
        • MAPT antibody
        • MAPTL antibody
        • MGC134287 antibody
        • MGC138549 antibody
        • MGC156663 antibody
        • Microtubule associated protein tau antibody
        • Microtubule associated protein tau isoform 4 antibody
        • Microtubule-associated protein tau antibody
        • MSTD antibody
        • Mtapt antibody
        • MTBT1 antibody
        • MTBT2 antibody
        • Neurofibrillary tangle protein antibody
        • Paired helical filament tau antibody
        • Paired helical filament-tau antibody
        • PHF tau antibody
        • PHF-tau antibody
        • PPND antibody
        • PPP1R103 antibody
        • Protein phosphatase 1, regulatory subunit 103 antibody
        • pTau antibody
        • RNPTAU antibody
        • TAU antibody
        • TAU_HUMAN antibody
        • Tauopathy and respiratory failure antibody
        • Tauopathy and respiratory failure, included antibody
        see all

      Images

      • Western blot - Anti-Tau (phospho S199) antibody (ab4749)
        Western blot - Anti-Tau (phospho S199) antibody (ab4749)
        Cell extracts from African green monkey kidney (CV-1) cells, stably expressing human four repeat tau and a protein phosphatase inhibitor, were resolved by SDS-PAGE on a 10% Tris-glycine gel. The proteins were transferred to nitrocellulose. Membranes were incubated with 0.50 µg/mL anti-phospho tau [pS199] (ab4749), following prior incubation in the absence (a) or presence of the peptide immunogen (b), or the nonphosphopeptide corresponding to the tau phosphopeptide (c). Cell extracts from African green monkey kidney (CV-1) cells, stably expressing human four repeat tau and a protein phosphatase inhibitor, were resolved by SDS-PAGE on a 10% Tris-glycine gel. The proteins were transferred to nitrocellulose. Membranes were incubated with 0.50 µg/mL anti-phospho tau [pS199] (ab4749), following prior incubation in the absence (a) or presence of the peptide immunogen (b), or the nonphosphopeptide corresponding to the tau phosphopeptide (c).

      Protocols

      • Peptide Competition Experiment
      • Western blotting protocol

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (3)

      Publishing research using ab4749? Please let us know so that we can cite the reference in this datasheet.

      ab4749 has been referenced in 3 publications.

      • Seo JS  et al. Transcriptome analyses of chronic traumatic encephalopathy show alterations in protein phosphatase expression associated with tauopathy. Exp Mol Med 49:e333 (2017). WB, IHC-P, ICC/IF ; Human . PubMed: 28524178
      • Casarejos MJ  et al. Natural cannabinoids improve dopamine neurotransmission and tau and amyloid pathology in a mouse model of tauopathy. J Alzheimers Dis 35:525-39 (2013). IHC-P ; Mouse . PubMed: 23478312
      • Rodríguez-Navarro JA  et al. Trehalose ameliorates dopaminergic and tau pathology in parkin deleted/tau overexpressing mice through autophagy activation. Neurobiol Dis 39:423-38 (2010). PubMed: 20546895

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